ATP Colorimetric/Fluorometric Assay Kit | TBS2010

Adenosine-5′-triphosphate (ATP) is a multifunctional nucleotide used in cells as a coenzyme. ATP is the main energy source for the majority of cellular functions. ATP also is critically involved in maintaining cell structure by facilitating assembly and disassembly of elements of the cytoskeleton. Tribioscience’s ATP Colorimetric and Fluorometric Assay kit is designed to be a robust, simple method that utilizes the phosphorylation of glycerol to generate a product that is easily quantified by colorimetric (OD = 570 nm) or fluorometric (Ex/Em = 535/590 nm) methods. The assay can detect down to 0.5 µM of ATP in various samples.

Applications

Direct Assays: as low as 0.5 µM of ATP in cells and other biological samples can be conducted.
Assay of enzymes that produce or degrade ATP.

Key Features

• Sensitive and accurate: Use 10 μL samples. Detection range 0.5-1000µM in 96-well plate assay.
• Simple and high-throughput: Simple procedure; takes less than 30 minutes. The kit is designed to be a robust method.

Storage and Handling

Store kit at -20°C. Shelf life of three months. Except Enzyme warm all of the components to room temperature before use. Briefly centrifuge all small vials prior to opening.

Standard Curve