ATP Sulfurylase, S. cerevisiae Recombinant | P1542

ATP Sulfurylase (EC No. EC2.7.7.4) catalyzes the first committed step in incorporating inorganic SO42- by converting ATP to adenylyl sulfate and pyrophosphate for the incorporation of inorganic SO42- into biomolecules. It is also a key enzyme used in pyrosequencing.

P1542 | ATP Sulfurylase, S. cerevisiae Recombinant DataSheet

Biomolecule/Target :

Synonyms: Sulfate Adenylyltransferase, Met3, ATPS, Methionine-requiring protein 3, Sulfate adenylate transferase

Alternates names: Sulfate Adenylyltransferase, Met3, ATPS, Methionine-requiring protein 3, Sulfate adenylate transferase

Taglines: A key enzyme used in pyrosequencing

NCBI Gene ID #: 853466

NCBI Gene Symbol: MET3

Gene Source: Saccharomyces cerevisiae

Accession #: P08536

Recombinant: TRUE

Source: E. coli

Purity by SDS-PAGE: >95%

Assay: SDS - PAGE

Purity:

Assay #2:

Endotoxin Level:

Activity (Specifications/test method): Enzyme activity assay: The activity of the enzyme is 100 U/mg. The enzyme’s activity was assayed using coupled hexokinase with ’s PicoProbe™ Glucose-6-Phosphate Dehydrogenase Activity Assay Kit (Cat. No. K751).

Biological activity: Enzyme activity assay: The activity of the enzyme is 100 U/mg. The enzyme’s activity was assayed using coupled hexokinase with ’s PicoProbe™ Glucose-6-Phosphate Dehydrogenase Activity Assay Kit (Cat. No. K751).

Results:

Binding Capacity:

Unit Definition: One unit is the amount of enzyme that will catalyze 1 umole of adenylyl sulfate (APS) to Adenosine Triphosphate (ATP) at pH 8.0 and 37 ÌŠC.

Molecular Weight: 57.7 kDa (amino acids: 1-511 with N-terminal His-Tag)

Concentration:

Appearance: Lyophilized protein

Physical form description: Lyophilized

Reconstitution Instructions: Reconstitute in 50 mM Tris pH 8, 30% glycerol, and 5 mM MgCl2 to a final concentration of 1 mg/ml.

Amino acid sequence: The sequence is from Saccharomyces cerevisiae. Full-length ATP Sulfurylase (aa 1-511 with N-Terminal His-Tag) (Accession# P08536)

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans