AurB (phospho Thr232) Antibody | 80-031

AurB (phospho Thr232) Antibody | 80-031 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: N/A

Immunogen: AurB (phospho Thr232) antibody was raised against a peptide sequence around phosphorylation site of threonine 232 (R-K-T (p) -M-C) derived from Human AurB.

Research Area: Phospho-Specific

Tested Application: WB

Application: Western Blot: 1:500~1:1000

Specificiy: The antibody detects endogenous level of AurB only when phosphorylated at threonine 232.

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: 39 kDa

Validation: N/A

Isoform: N/A

Purification: Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+) , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Concentration: 1 mg/mL

Storage Condition: Store antibody at -20˚C for up to one year.

Alternate Name: AIK2, AIM-1, ARK2, AURKB, Aurora-B

User Note: N/A

BACKGROUND: Serine/threonine-protein kinase component of the chromosomal passenger complex (CPC) , a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Involved in the bipolar attachment of spindle microtubules to kinetochores and is a key regulator for the onset of cytokinesis during mitosis. Required for central/midzone spindle assembly and cleavage furrow formation. AURKB phosphorylates the CPC complex subunits BIRC5/survivin, CDCA8/borealin and INCENP. Phosphorylation of INCENP leads to increased AURKB activity.