Azidothymidine | B1113

Azidothymidine is a nucleoside reverse transcriptase inhibitor that, following triphosphorylation by thymidine kinase, potently blocks replication of HIV (EC₅₀ = 3 nM) with low cytotoxicity (CC₅₀ > 5 μM). Displays 100-fold selectivity for HIV reverse transcriptase over DNA polymerase α. Suppresses HIV-1 replication and enhances cell viability in HIV-1 infected T cells. Antiretroviral agent. Also decreases CRISPR-mediated homology directed repair (HDR) efficiency.

Azidothymidine is a nucleoside reverse transcriptase inhibitor that, following triphosphorylation by thymidine kinase, potently blocks replication of HIV (EC₅₀ = 3 nM) with low cytotoxicity (CC₅₀ > 5 μM). Displays 100-fold selectivity for HIV reverse transcriptase over DNA polymerase α. Suppresses HIV-1 replication and enhances cell viability in HIV-1 infected T cells. Antiretroviral agent. Also decreases CRISPR-mediated homology directed repair (HDR) efficiency.

B1113 | Azidothymidine DataSheet

Alternate Name/Synonyms: 3'-Azido-3'-deoxythymidine; AZT; Zidovudine

Appearance: White solid

Formulation: N/A

CAS Number: 30516-87-1

Structure Available?: Yes

Peptide sequence: N/A

Salt Form: No

Molecular Formula: C₁₀H₁₃N₅O₄

Molecular Weight: 267.24

Cell-Permeable?: Yes

Purity: ≥98% by HPLC

Solubilities: DMSO (>25 mg/ml) or H2O (>10 mg/ml)

Handling: Protect from air and moisture

Country of Origin: USA

Tag Line: A nucleoside reverse transcriptase inhibitor and homology directed repair (HDR) blocker

MDL Number: MFCD00006536

PubChem CID: 35370

SMILES: CC1=CN(C(=O)NC1=O)C2CC(C(O2)CO)N=[N+]=[N-]

InChi: InChI=1S/C10H13N5O4/c1-5-3-15(10(18)12-9(5)17)8-2-6(13-14-11)7(4-16)19-8/h3,6-8,16H,2,4H2,1H3,(H,12,17,18)/t6-,7+,8+/m0/s1

InChi Key: HBOMLICNUCNMMY-XLPZGREQSA-N