B-raf Antibody | 7379

B-raf Antibody | 7379 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse

Homology: Predicted species reactivity based on immunogen sequence: Rat: (100%) , Chicken: (100%)

Immunogen: Anti-B-raf antibody (7379) was raised against a peptide corresponding to 19 amino acids near the center of human B-raf.
The immunogen is located within amino acids 330 - 380 of B-raf.

Research Area: Cancer, Cell Cycle

Tested Application: E, WB, IHC-P, IF

Application: WB: 0.5-2 μg/mL; IHC: 2.5 μg/mL; IF: 20 μg/mL.
Antibody validated: Western Blot in human and mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: This antibody will not cross-react with C-raf.

Positive Control 1: Cat. No. 1303 - Human Brain Tissue Lysate

Positive Control 2: Cat. No. 11-801 - Human Small Intestine Tissue Slide

Positive Control 3: Cat. No. 1224 – Daudi Cell Lysate

Positive Control 4: Cat. No. 1204 – K562 Cell Lysate

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 94kD
Observed: 94 kD

Validation:

Independent Antibody Validation in Cell lines (Figure 2) shows similar B-raf expression profile in human and mouse cell lines detected by two independent anti-B-raf antibodies that recognize different epitopes, 5777 against central domain and 7379 against a slight different central domain.  B-raf proteins are detected in the most tested cell lines at different expression levels by the two independent antibodies. 

Isoform: Human B-raf has one isoform (766aa, 84kD) . Mouse B-raf has 2 isoforms, including isoform 1 (804aa, 89kD) and isoform 2 (329aa, 37kD) . Rat B-raf a has one isoform (712aa, 80kD) . 7379 can detect in human B-raf and mouse isoform 1.

Purification: B-raf Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: B-raf Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: B-raf antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: B-raf Antibody: NS7, BRAF1, RAFB1, B-RAF1, Proto-oncogene B-Raf

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: B-raf Antibody: B-raf belongs to the raf/mil family of serine/threonine protein kinases and plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. The Ras/Raf/MEK/ERK and Ras/PI3K/PTEN/Akt pathways interact with each other to regulate growth and in some cases tumorigenesis. Mutations in B-raf have been associated with several cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung, leading to speculation on the possibility of B-raf as a therapeutic target for treating cancers. Mutations in this gene have also been associated with cardiofaciocutaneous syndrome (CFCS) , a disease characterized by heart defects, mental retardation and a distinctive facial appearance.