Bacterial Vaginosis RT-PCR Quant (CE) | B74-FRT

Bacterial Vaginosis RT-PCR Quant (CE) | B74-FRT is available for delivery

Multiplex Real Time PCR kit for quantitative detection of Gardnerella vaginalis, Atopobium vaginae, Lactobacillus spp. and total bacteria quantity

Storage & Shipping :

The kit can be shipped at 2-8°C for 3-4 days but should be stored at 2-8°C and -20°C immediately on receipt.

Bacterial Vaginosis RT-PCR Quant (CE) B74-FRT DataSheet

INTRODUCTION

Bacterial vaginosis (BV) is considered to be the most common cause of vaginal inflammation among both pregnant and non-pregnant women and prevalence between 4.9% and 36.0% have been reported from European and American studies. It previously was called nonspecific vaginitis or Gardnerella-associated vaginitis. The adult human vagina is a complex ecosystem containing an abundance of microorganisms. In women of childbearing age this system is dominated by Lactobacillus spp., a genus of gram-positive, nonmotile rod-like bacteria, a defining characteristic of which is the ability to grow in acid media and tolerate acid conditions (pH < 4.5); lactobacilli also ferment carbohydrates to produce lactic acid and produce H2O2 which provides a natural defence against Gardnerella vaginalis.

In bacterial vaginosis (BV) the balance of flora is changed with reduced numbers of lactobacilli (normal concentration 106 – 1010 CFU/ml) and an increase in numbers of other bacteria. Today more than 200 BV-associated microorganisms are described (mostly anaerobic). The studies in the recent years show that Gardnerella vaginalis plays the key role in development of bacterial vaginosis because of several reasons:

• it is detected almost in all patients with bacterial vaginosis,
• it has the strongest pathogenic potential among all the described BV-assosiated bacteria,
• it has significant adhesiveness,
• it forms biofilm,
• it produces cytolisin (vaginolisin), sialidase.

G. vaginalis is virtually always present at high concentrations in women who have BV but is also detected frequently in normal women and in some cases the concentration of Gardnerella vaginalis can reach 107-108 CFU/ml also in absence of BV, so the most important maker of BV is the ratio of logarithm concentration Lactobacillus spp and G. vaginalis.

Another marker of bacterial vaginosis is Atopobium vaginae. It is a highly specific microorganism for this syndrome. Atopobium vaginae is a highly metronidazole resistant microorganism. Thus, its detection can cause the change of treatment strategy. The clinical significance of studying vaginal flora is that it helps determine the quantity of microorganisms and assess the ratio between the different groups of conditionally pathogenic microorganisms and the normal flora.

INTENDED USE

Bacterial Vaginosis Real-TM Quant kit is for the quantitative detection of Gardnerella vaginalis, Atopobium vaginae, Lactobacillus spp. and total bacteria quantity in the vaginal biotope. Bacterial Vaginosis Real-TM Quant kit allows to value the ratio among the total bacteria quantity, lactobacilli and other facultative and anaerobic species (Gardnerella vaginalis, Atopobium vaginae) in the vaginal biotope; the total quantity of bacteria is useful to value that the collected material is sufficient.

The logarithmic ratio between Lactobacillus spp. and the total quantity of bacteria and also between Lactobacillus spp. and facultative pathogen microorganisms (Gardnerella vaginalis andAtopobium vaginae) allows to detect with high precision bacterial vaginosis disease related to normal vaginal microflora suppression (Lactobacillus spp.) and substitution with facultative pathogen microorganisms (Gardnerella vaginalis and Atopobium vaginae). The use of Bacterial Vaginosis Real-TM Quant kit allows to monitor vaginal environment condition and to check the therapy efficiency.

PRINCIPLE OF ASSAY

Kit Bacterial Vaginosis Real-TM Quant is based on two major processes: isolation of DNA from specimens and Real Time amplification. DNA is extracted from the specimens, amplified in Real Time PCR and detected using fluorescent reporter dye probes specific for DNA Gardnerella vaginalis, DNA Atopobium vaginae, DNA Lactobacillus spp. and DNA of Bacteria.