BAFF Antibody | 2221

BAFF Antibody | 2221 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: N/A

Immunogen: Anti-BAFF antibody (2221) was raised against a peptide corresponding to 16 amino acids near the carboxy terminus of human BAFF.
The immunogen is located within the last 50 amino acids of BAFF.

Research Area: Chemokines & Cytokines, Cancer

Tested Application: E, WB, ICC, IF, IHC

Application: WB: 0.25-1 μg/mL; ICC: 1 μg/mL; IF: 20 μg/mL.
Antibody validated: Western Blot in human, mouse and rat samples; Immunocytochemistry in human samples; Immunofluorescence in human samples; Immunohistochemistry in human and mouse samples. All other applications and species not yet tested.

Specificiy: N/A

Positive Control 1: Cat. No. 1209 - HL60 Cell Lysate

Positive Control 2: Cat. No. 1406 - Mouse Spleen Tissue Lysate

Positive Control 3: Cat. No. 17-009 - HL-60 Cell Slide

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 31kD
Observed: 35 kD

Validation:

Recombinant Protein Test (Figure 3) : Anti-BAFF antibodies (2221) detected human BAFF recombinant protein at different concentrations.

Regulated expression validation (Figure 6-8) : BAFF expression detected by anit-BAFF antibodies (2221) was up-regulated  in patients with myloma or Graves’ disease or in murine transplanted heart.

Isoform: Human BAFF has 3 isoforms, including isoform 1 (285aa, 31kD) , isoform 2 (266aa, 29kD) and isoform 3 (164aa, 17.6kD) . Mouse BAFF has 2 isoforms, including isoform 1 (309aa, 34kD) and isoform 2 (290aa, 32kD) . Rat BAFF has only one isoform (274aa, 30kD) . 2221 can detect two longer human isoforms, mouse and rat.

Purification: BAFF Antibody is Ion exchange chromatography purified.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: BAFF Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: BAFF antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: BAFF Antibody: DTL, BAFF, BLYS, CD257, TALL1, THANK, ZTNF4, TALL-1, TNFSF20, UNQ401/PRO738, Tumor necrosis factor ligand superfamily member 13B, B lymphocyte stimulator

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: BAFF Antibody: Members in the TNF superfamily regulate immune responses and induce apoptosis. A novel member in the TNF family was recently identified by several groups and designated BAFF (for B cell Activating Factor belonging to the TNF Family) , BLyS (for B Lymphocyte Stimulator) , TALL-1 (for TNF- and ApoL-related Leukocyte-expressed Ligand) , and THANK (for TNF Homologue that Activate Apoptosis, NF-κB and c-jun N-terminal Kinase) . BAFF/BLyS was characterized as a B cell activator since it induced B cell proliferation and immunoglobulin secretion. Three receptors for BAFF were recently identified and designated TACI, BCMA and BAFF-R. BAFF and its receptors are essential for B cell development, survival, and humoral immune responses. BAFF is involved in the development of autoimmune diseases including systemic lupus erythaematosus and rheumatoid arthritis.