BAK1 | Brassinosteroid insensitive 1-associated receptor kinase 1 | AS12 1858

BAK1 | Brassinosteroid insensitive 1-associated receptor kinase 1 | AS12 1858 | Gentaur UK, US & Europe Distribution

Immunogen: KLH-conjugated peptide, chosen from Arabidopsis thaliana Bak1 sequence, TAIR: AT4G33430 UniProt: Q94F62

Host: Rabbit

Conjugation: N/A

Clonality: Polyclonal

Isotype: N/A

Purity: Immunogen affinity purified serum in PBS pH 7.4.

Format: Lyophilized

Tested Application: Immunoprecipitation (IP), Western blot (WB)

Related Products: AS12 1859 | Anti-BRI1 | BRASSINOSTEROID INSENSITIVE 1, rabbit antibodiesAS12 1857 | Anti-FLS2 | FLAGELLIN-SENSITIVE 2, rabbit antibodiesAS16 3203 | Anti-BIN2 | brassinosteroid insensitive 2, rabbit antibodiesAS16 3204 | Anti-SOBIR1 | supressor of BIR1, rabbit antibodiesPlant protein extraction buffer Secondary antibodies

Recommended Dilutions: 2 µl/50 µl of Protein G agarose, 1 : 5000 (WB)

Molecular weight: 68 | 70 kDa

Confirmed Reactivity: Arabidopsis thaliana, Solanum lycopersicum

Predicted Reactivity: Thelungiella halophila Species of your interest not listed? Contact us

Not reactive in: Hordeum vulgare, Nicotiana benthamiana, Oryza sativa

Additional Information: Extra Information on CE extraction buffer: CE buffer does not need to be made freshly everytime. Aliquots can be kept at -20°C. Na2MoO4 and NaF are phosphatase inhibitors, included to prevent lose phosphorylation form our protein of interest during extraction. EDTA chelates metal ions and thus inhibits many enzymes which need metal ions as co-factors and inhibits the action of proteases. Protease inhibitor coctail is Sigma product number P 9599 which is used in dilution 1: 100.Antibody is reconizing AtBAK1-Myc in total extracts.

Background: BAK1 (Brassinosteroid insensitive 1-associated receptor kinase 1) controls expression of genes associated with innate immunity in the absence of pathogens or elicitors. Phosphorylated Brl1. Involved in programmed cell death (PCD) . Subcellular localization: cell membrane. Alternative names: BRASSINOSTEROID INSENSITIVE 1-associated receptor kinase 1, BAK1, BRI1-ASSOCIATED RECEPTOR KINASE, RKS10, RECEPTOR KINASES LIKE SERK 10, SERK3, SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE 3, ELG, ELONGATED, ATSERK3, SOMATIC EMBRYOGENESIS RECEPTOR-LIKE KINASE 3, ATBAK1.

Reconstitution: For reconstitution add 50 µl of sterile water

Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles, Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

TAIR Nnumbre: AT4G33430

Category: Brassinosteroids/regulation

Research Area: Plant Biology