BAP31 Antibody | 3675

BAP31 Antibody | 3675 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: N/A

Immunogen: Anti-BAP31 antibody (3675) was raised against a peptide corresponding to 16 amino acids near the center of human BAP31.
The immunogen is located within amino acids 130 - 180 of BAP31.

Research Area: Signal Transduction

Tested Application: E, WB, IHC-P

Application: WB: 0.5-2 μg/mL; IHC: 10 μg/mL.
Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in rat samples. All other applications and species not yet tested.

Specificiy: N/A

Positive Control 1: Cat. No. 1462 - Rat Lung Tissue Lysate

Positive Control 2: Cat. No. 1204 - K562 Cell Lysate

Positive Control 3: Cat. No. 1223 - Caco-2 Cell Lysate

Positive Control 4: Cat. No. 1282 - 3T3/NIH Cell Lysate

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 28 or 34kD
Observed: 28 kD

Validation:

Independent Antibody Validation in Cell lines (Figure 2) shows similar BAP31 expression profile in human cell lines detected by two independent anti-BAP31 antibodies that recognize different epitopes, 3675 against central domain and 64-237, against recombinant protein.  BAP31 proteins are detected in all tested cell lines at different expression levels by the two independent antibodies.

Isoform: Human BAP31 has 2 isoforms, including isoform 1 (246aa, 28kD) and isoform 2 (313aa, 35kD) . Mouse BAP31 has one isoform (245aa, 28kD) and Rat BAP31 also has one isoform (245aa, 28kD) . 3675 can detect human, mouse and rat.

Purification: BAP31 Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: BAP31 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: BAP31 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: BAP31 Antibody: CDM, DDCH, BAP31, 6C6-AG, DXS1357E, B-cell receptor-associated protein 31, 6C6-AG tumor-associated antigen, BCR-associated protein 31

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: BAP31 Antibody: Bap31 and the related protein Bap29 are endoplasmic reticulum (ER) and ER-vesicle membrane proteins and members of the B-cell receptor-associated protein family. These two proteins are highly homologous and can form homo- and heterodimers. Bap31 is thought to be involved in the intracellular trafficking of several molecules such as MHC Class I molecules and CD11b/CD18. Bap31 may also play a role in the initiation of ER stress-induced apoptosis through its association with caspase-8 via a death effector domain in its cytoplasmic tail, possibly through the promotion of membrane fragmentation and the release of cytochrome c from mitochondria. Bap31 itself contains two caspase cleavage sites and is cleaved during apoptosis. The p20 fragment of Bap31, when expressed ectopically, is also a potent inducer cell death.