Bcl-2 Antibody | 3337

Bcl-2 Antibody | 3337 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse

Homology: Predicted species reactivity based on immunogen sequence: Bovine: (100%) , Rat: (93%) , Chicken: (86%)

Immunogen: Anti-Bcl-2 antibody (3337) was raised against a peptide corresponding to 14 amino acids near the center of human Bcl-2.
The immunogen is located within amino acids 80-130 of Bcl-2.

Research Area: Apoptosis, Neuroscience, Autophagy, Cancer

Tested Application: E, WB, ICC

Application: WB: 1-4μg/mL; ICC: 2 μg/mL.
Antibody validated: Western Blot in human and mouse samples; Immunocytochemistry in mouse samples. All other applications and species not yet tested.

Specificiy: N/A

Positive Control 1: Cat. No. 1288 - A20 Cell Lysate

Positive Control 2: Cat. No. 1205 - Jurkat Cell Lysate

Positive Control 3: Cat. No. 1208 - THP-1 Cell Lysate

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 26kD
Observed: 26 kD

Validation:

Independent Antibody Validation in Cell lines (Figure 2) shows similar Bcl-2 expression profile in human and mouse cell lines detected by two independent anti-Bcl-2 antibodies that recognize different epitopes, 3335 against N-terminus domain and 3337 against central domain.  Bcl-2 proteins are detected in several tested cell lines at different expression levels by the two independent antibodies. 

Isoform: Human Bcl-2 has 2 isoforms, including isoform 1 (239aa, 26kD) , and isoform 2 (205aa, 22kD) . Mouse Bcl-2 has 2 isoforms, including isoform 1 (236aa, 26kD) , and isoform 2 (199aa, 22kD) . Rat Bcl-2 has one isoform (236aa, 27kD) . 3337 can detect all isoforms of human, mouse and rat

Purification: Bcl-2 Antibody is Ion exchange chromatography purified.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Bcl-2 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: Bcl-2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: Bcl-2 Antibody: Bcl-2, PPP1R50, Apoptosis regulator Bcl-2

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: Bcl-2 Antibody: Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Disruption of this process has been implicated in a variety of diseases such as cancer. Bcl-2 is the founding member of a family of over 20 proteins that are critical regulators of apoptosis. These can be divided into two classes: those that inhibit apoptosis and those that promote cell death. Bcl-2 is an inner mitochondrial membrane protein that inhibits apoptosis. It is thought to act by interacting with pro-apoptotic Bcl-2 family members such as Bak and Bad. Overexpression of Bcl-2 has been linked to human cancers such as B-cell lymphoma and prostate cancer.