Description
BCLAF1 Antibody | 8373 | Gentaur UK, US & Europe Distribution
Host: Rabbit
Reactivity: Human, Mouse, Rat
Homology: N/A
Immunogen: BCLAF1 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human BCLAF1.
The immunogen is located within amino acids 820 - 870 of BCLAF1.
Research Area: Stem Cell, Apoptosis
Tested Application: E, WB, IHC-P
Application: BCLAF1 antibody can be used for detection of BCLAF1 by Western blot at 0.5 - 1 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in mouse samples. All other applications and species not yet tested.
Specificiy: BCLAF1 antibody is human, mouse and rat reactive. At least four isoforms of BCLAF1 are known to exist; this antibody will detect all but isoform 2.
Positive Control 1: Cat. No. 1282 - 3T3 (NIH) Cell Lysate
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: Predicted: 101 kDa
Observed: 105 kDa
Validation: N/A
Isoform: N/A
Purification: BCLAF1 antibody is affinity chromatography purified via peptide column.
Clonality: Polyclonal
Clone: N/A
Isotype: IgG
Conjugate: Unconjugated
Physical State: Liquid
Buffer: BCLAF1 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Condition: BCLAF1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.
Alternate Name: BCL2-associated transcription factor 1
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: The BCL2-associated transcription factor 1 (BCLAF1) is a transcriptional repressor that interacts with several members of the BCL2 family of proteins. Overexpression of this protein induces apoptosis, which can be suppressed by co-expression of BCL2 proteins (1) . BCLAF1 localizes to dot-like structures throughout the nucleus, and redistributes to a zone near the nuclear envelope in cells undergoing apoptosis. (2) . More recent studies have suggested BCLAF1 plays roles in processes not associated with actions of the BCL2 family members, including lung development, T cell activation, and post-transcriptional processes that affect mRNA metabolism (3) .