Benzonase, E. Coli Recombinant | 7680

Golden Nuclease is a recombinant form of Serratia macescens extracellular endonuclease produced in Escherichia coli. Golden Nuclease is a homodimer with monomer molecular masses about 30 kDa. Two disulfide bonds found in the nuclease are crucial to its activity and stability. The enzyme is a non-specific nuclease with high specific activity, which degrades both single- and double-stranded nucleic acids in any form (single stranded, double stranded, linear, circular and supercoiled). It hydrolyzes internal phosphodiester bonds present between the nucleotides to 5'-phosphorylated oligonucleotides of 3-8 bases in length.

7680 | Benzonase E. Coli Recombinant DataSheet

Biomolecule/Target: Benzonase

Synonyms: Endoribonuclease

Alternates names: Fatty acid-binding protein 1 liver, L-FABP, FABPL, FABP-1, FABP1, Z-protein.

Taglines: A non-specific, highly active nuclease, which degrades both single- and double-stranded nucleic acids.

NCBI Gene ID #: 2168

NCBI Gene Symbol: FABP1

Gene Source: E Coli

Accession #: AAH22287

Recombinant: Yes

Source: E. coli

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: Test in process

Results: N/A

Binding Capacity: N/A

Unit Definition: One unit is defined as the change of A260 = 1 (equivalent to 37 g of DNA) when sonicated salmon sperm DNA is digested with the enzyme for 30 min at 37°C at pH 8.0.

Molecular Weight: 19 kDa

Concentration: N/A

Appearance: Liquid

Physical form description: 250 U/µl in 50% glycerol

Reconstitution Instructions: Reconstitute in HO to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers

Amino acid sequence: N/A