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Benzonase, E. Coli Recombinant | 7680
- SKU:
- 26-7680-GEN
- Availability:
- Usually shipped in 5 working days
Description
Golden Nuclease is a recombinant form of Serratia macescens extracellular endonuclease produced in Escherichia coli. Golden Nuclease is a homodimer with monomer molecular masses about 30 kDa. Two disulfide bonds found in the nuclease are crucial to its activity and stability. The enzyme is a non-specific nuclease with high specific activity, which degrades both single- and double-stranded nucleic acids in any form (single stranded, double stranded, linear, circular and supercoiled). It hydrolyzes internal phosphodiester bonds present between the nucleotides to 5'-phosphorylated oligonucleotides of 3-8 bases in length.
7680 | Benzonase E. Coli Recombinant DataSheet
Biomolecule/Target: Benzonase
Synonyms: Endoribonuclease
Alternates names: Fatty acid-binding protein 1 liver, L-FABP, FABPL, FABP-1, FABP1, Z-protein.
Taglines: A non-specific, highly active nuclease, which degrades both single- and double-stranded nucleic acids.
NCBI Gene ID #: 2168
NCBI Gene Symbol: FABP1
Gene Source: E Coli
Accession #: AAH22287
Recombinant: Yes
Source: E. coli
Purity by SDS-PAGEs: 98%
Assay: SDS-PAGE
Purity: N/A
Assay #2: HPLC
Endotoxin Level: N/A
Activity (Specifications/test method): N/A
Biological activity: Test in process
Results: N/A
Binding Capacity: N/A
Unit Definition: One unit is defined as the change of A260 = 1 (equivalent to 37 g of DNA) when sonicated salmon sperm DNA is digested with the enzyme for 30 min at 37°C at pH 8.0.
Molecular Weight: 19 kDa
Concentration: N/A
Appearance: Liquid
Physical form description: 250 U/µl in 50% glycerol
Reconstitution Instructions: Reconstitute in HO to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers
Amino acid sequence: N/A