Beta-lactaminase, Human Recombinant | P1196

AmpC, also known as Beta-lactamase, is the most widespread resistance mechanism to beta-lactam antibiotics, such as the penicillins and the cephalosporins. These antibiotics have a common element in their molecular structure: a four-atom ring known as a beta-lactam. The lactamase enzyme breaks that ring open, deactivating the molecule's antibacterial properties. Recombinant E.coli beta-lactamase protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

P1196 | Beta-lactaminase Human Recombinant DataSheet

Biomolecule/Target: ampC

Synonyms: ampC, ampA, Cephalosporinase

Alternates names: Fatty acid-binding protein heart, H-FABP, Heart-type fatty acid-binding protein, Muscle fatty acid-binding protein, M-FABP, Mammary-derived growth inhibitor, MDGI, FABP3, FABP11, O-FABP.

Taglines: Serine beta-lactamase with a substrate specificity for cephalosporins

NCBI Gene ID #: 2170

NCBI Gene Symbol: FABP3

Gene Source: Human

Accession #: P05413

Recombinant: Yes

Source: E.coli

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): Measured by its ability to hydrolyze 1.0 µmole of Nitrocefin per minute at pH 7.0 at 37C.

Biological activity: Test in process

Results: >700 units/mg

Binding Capacity: N/A

Unit Definition: One unit will hydrolyze 1.0 µmole of Nitrocefin per minute at pH 7.0 at 37C.

Molecular Weight: 15.1 kDa

Concentration: N/A

Appearance: Liquid

Physical form description: In 20 mM Tris-HCl buffer (pH8.0) containing 10% glycerol

Reconstitution Instructions: Reconstitute in HO to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers

Amino acid sequence: N/A