Betacellulin, Bovine Recombinant | P1588

BTC is a potent mitogen for retinal pigment epithelial cells and vascular smooth muscle cells. The effects of betacellulin are probably mediated by the EGF receptor and other related receptors. BTC is a growth factor that binds to EGFR, ERBB4 and other EGF receptor family members. It is a potent mitogen for retinal pigment epithelial cells and vascular smooth muscle cells.

P1588 | Betacellulin, Bovine Recombinant DataSheet

Biomolecule/Target :

Synonyms: BTC

Alternates names: BTC

Taglines: Growth factor that binds to EGFR, ERBB4 and other EGF receptor family members.

NCBI Gene ID #: 280737

NCBI Gene Symbol: BTC

Gene Source: Bovine

Accession #: Q9TTC5

Recombinant: TRUE

Source: E. coli

Purity by SDS-PAGE: >95%

Assay:

Purity:

Assay #2:

Endotoxin Level:

Activity (Specifications/test method): The ED50, calculated by the dose-dependent proliferation of murine BALB\C 3T3 cells (measured by 3H-thymidine uptake) is < 10.0 ng/ml, corresponding to a Specific Activity 100,000 units/mg.

Biological activity:

Results:

Binding Capacity:

Unit Definition:

Molecular Weight: 9003 Dalton

Concentration:

Appearance: Lyophilized

Physical form description: Lyophilized after extensive dialysis against 50mM acetic acid.

Reconstitution Instructions: It is recommended to reconstitute the lyophilized BTC Bovine in sterile 18MΩ-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Amino acid sequence: The protein has 80 amino acids. The sequence of the first five N-terminal amino acids was determined and was found to be Asp-Gly-Asn-Ser-Thr.

Handling: Centrifuge the vial prior to opening.

Usage: For Research Use Only! Not to be used in humans