Betatrophin Antibody | 7619

Betatrophin Antibody | 7619 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: N/A

Immunogen: Betatrophin antibody was raised against a 16 amino acid peptide near the amino terminus of human Betatrophin.
The immunogen is located within amino acids 40 - 90 of Betatrophin.

Research Area: Obesity

Tested Application: E, WB, IHC-P, IF

Application: Betatrophin antibody can be used for detection of Betatrophin by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples; Immunohistochemistry in mouse and rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.

Specificiy: Betatrophin antibody is human, mouse and rat reactive. At least three isoforms of Betatrophin are known to exist; this antibody will detect the two largest isoforms.

Positive Control 1: Cat. No. 1464 - Rat Liver Tissue Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 22 kDa
Observed: 22 kDa

Validation: N/A

Isoform: N/A

Purification: Betatrophin antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Betatrophin antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: Betatrophin antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year.

Alternate Name: Betatrophin Antibody: RIFL, TD26, ANGPTL8, PRO1185, PVPA599, Angptl8, UNQ599/PRO1185, Betatrophin, Angiopoietin-like protein 8

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: The Betatrophin protein, also known as the hepatocellular carcinoma-associated protein TD26, is an adipocyte- and liver-enriched insulin targeted gene that is thought to play a role in lipid metabolism be regulating serum triglyceride levels (1, 2) . Betatrophin is closely related to the angiopoietin-like (ANGPTL) protein family. Betatrophin mRNA increases in the cold environment and after treatment with a high-fat diet, while its mRNA levels decrease in response to fasting, suggesting that it may play a role in mediating thermogenesis and energy homeostasis (3) .