BICD1 Antibody | 4663

BICD1 Antibody | 4663 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: N/A

Immunogen: BICD1 antibody was raised against a 15 amino acid synthetic peptide from near the carboxy terminus of human BICD1.
The immunogen is located within the last 50 amino acids of BICD1.

Research Area: Neuroscience

Tested Application: E, WB, ICC, IF

Application: BICD1 antibody can be used for detection of BICD1 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 10 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Specificiy: At least two isoforms of BICD1 are known to exist; this antibody will only recognize the larger isoform.

Positive Control 1: Cat. No. 1224 - Daudi Cell Lysate

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: BICD1 Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: BICD1 Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: BICD1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: BICD1 Antibody: BICD, Protein bicaudal D homolog 1, Bic-D 1

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: BICD1 Antibody: BICD1 was initially identified as the human homolog to Drosophila Bicaudal-D protein that forms part of the cytoskeleton and mediates the correct sorting of mRNAs for oocyte- and axis-determining factors during oogenesis. BICD1 can bind to dynein-dynactin and co-localizes with Rab6A on the trans-Golgi network and cytoplasmic vesicles, suggesting BICD1 regulates COPI-independent Golgi-ER transport. BICD1 will also interact with Rab6B, a Rab6 isoform that is expressed primarily in brain. Live cell imaging showed bi-directional movement of Rab6B structures in SK-N-SH neurites, indicating that BICD1 is involved in the Rab6B regulation of retrograde cargo transport in neuronal cells.