Description
BID Antibody | 45-021 | Gentaur UK, US & Europe Distribution
Host: Goat
Reactivity: Human
Homology: N/A
Immunogen: The immunogen for this antibody is: C-TYVRSLARNGMD
Research Area: Apoptosis, Cancer
Tested Application: E, WB, IF
Application: Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 26+24kDa bands observed in A431 cell lysates (calculated MW of 26.8kDa according to NP_932070.1 and 22.0kDa according to NP_001187.1) . Recommended concentration: 0.1-0.3ug/ml. Primary incubation 1 hour at room temperature.Immunofluorescence: Strong expression of the protein seen in the cytoplasm of MCF7 cells. Recommended concentration: 10ug/ml.
Specificiy: This antibody is expected to recognise all three reported isoforms (as represented by NP_932070.1; NP_001187.1; NP_932071.1)
Positive Control 1: Cat. No. 1202 - A431 Cell Lysate
Positive Control 2: N/A
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality: Polyclonal
Clone: N/A
Isotype: N/A
Conjugate: Unconjugated
Physical State: Liquid
Buffer: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration: 500 ug/mL
Storage Condition: Aliquot and store at -20˚C. Minimize freezing and thawing.
Alternate Name: BID, BH3 interacting domain death agonist, HGNC:1050, MGC15319, MGC42355, BH3-interacting domain death agonist, Human BID coding sequence, apoptic death agonist, FP497, BID isoform ES (1b) , BID isoform L (2) , BID isoform Si6, OTTHUMP00000196197, desmocollin type 4
User Note: N/A
BACKGROUND: N/A