Description
Bim Antibody [1C2C8] | PM-4819 | Gentaur UK, US & Europe Distribution
Host: Mouse
Reactivity: Human, Mouse
Homology: N/A
Immunogen: Mouse monoclonal Bim antibody was raised against a recombinant protein corresponding to amino acids 2 to 198 of human Bim.
Research Area: Apoptosis, Cancer
Tested Application: E, WB, ICC, IF
Application: Bim antibody can be used for detection of Bim by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificiy: N/A
Positive Control 1: Cat. No. 95-107 - Bim Recombinant Protein
Positive Control 2: Cat. No. 17-004 - K-562 Cell Slide
Positive Control 3: N/A
Positive Control 4: N/A
Positive Control 5: N/A
Positive Control 6: N/A
Molecular Weight: N/A
Validation: N/A
Isoform: N/A
Purification: Bim Monoclonal Antibody is immunoaffinity chromotography purified IgG.
Clonality: Monoclonal
Clone: 1C2C8
Isotype: IgG1
Conjugate: Unconjugated
Physical State: Liquid
Buffer: Bim Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Condition: Bim monoclonal antibody can be stored at -20˚C, stable for one year.
Alternate Name: Bim Antibody [1C2C8] : BAM, BIM, BOD
User Note: Optimal dilutions for each application to be determined by the researcher.
BACKGROUND: Bim Monoclonal Antibody: Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3 domain containing pro-apoptotic proteins, including Bad, Bax, Bid, Bik, and Hrk, form a growing subclass of the Bcl-2 family. Bim is another BH3 domain containing protein which can induce apoptosis. Bim interacts with diverse members in the pro-survival Bcl-2 sub-family including Bcl-2, Bcl-xL and Bcl-w. The messenger RNA of Bim is ubiquitously expressed in multiple tissues and cell lines.