Biotin Ligase (BirA), Recombinant | P1674

Biotin Ligase (BirA), Recombinant | P1674

Description: Biotin-protein ligase (EC 6.3.4.15) activates biotin to form biotinyl 5'-adenylate and transfers the biotin to biotin-accepting proteins. BirA is E coli biotin ligase that biotinylates specifically a lysine side chain within a 15-amino acid acceptor peptide (also known as Avi-tag).

Biomolecule/Target:

Synonyms: BirA, Biotin operon repressor protein; Biotin holoenzyme synthetase; biotin-(acetyl-CoA carboxylase) synthetase

Alternates names:

Taglines: An enzyme that transfers biotin to biotin-accepting proteins

NCBI Gene ID #: 948469

NCBI Gene Symbol: E.Coli

Accession #: P06709

Recombinant: False

Source: E. coli

Purity by SDS-PAGE: ≥ 95% by SDS-PAGE

Assay:

Purity:

Assay #2:

Endotoxin Level:

Activity (Specifications/test method): ≥ 30 mU/mg

Biological activity:

Results:

Binding Capacity:

Unit Definition: One unit of BirA is defined as the amount of enzyme that catalyzes the biotinylation of 1 µmol of Avi-tagged protein (CD40L-Avi) per minute under the assay conditions, where 50% of CD40L-Avi is biotinylated in 60 min at 30 °C in the reaction buffer containing 5 mM MgCl2, 2 mM ATP, 150 µM biotin and 50 µM substrate in PBS and monitored on an SDSPAGE.

Molecular Weight: 37.5 kDa (with N-terminal His tag)

Concentration:

Appearance: Sterile Filtered colorless solution

Physical form description: Liquid

Reconstitution Instructions:

Amino acid sequence: Amino acids 2 to 321

Handling: Centrifuge the vial prior to opening.

Usage: