BirA (mutated/TurboID) - Biotin conjugated 40 µg | AS20 4440B

BirA (mutated/TurboID) - Biotin conjugated 40 µg | AS20 4440B | Gentaur UK, US & Europe Distribution

Immunogen: Recombinant mutated BirA protein from E.coli produced using the following plasmid: TurboID-His6_pET21a, (Plasmid #107177) . Expression was done in a vector that allowed for the generation of an untagged protein (without HIS6tag) .

Host: Rabbit

Conjugation: Biotin

Clonality: Polyclonal

Isotype: N/A

Purity: Immunogen affinity purified serum in PBS pH 7.4, conjugated to biotin.

Format: Liquid

Tested Application: N/A

Related Products: Collection of antibodies to various epitope Tags

Recommended Dilutions: 1 : 5000 (WB)

Molecular weight: Depends upon fusion partner

Confirmed Reactivity: BirA (mutated/TurboID)

Predicted Reactivity: N/A

Not reactive in: No confirmed exceptions from predicted reactivity are currently known

Additional Information: N/A

Background: Tagging a protein with TurboID allows studying protein interactions in different types of cells and organs and developmental stages. This is a suitable tool for proximity labelling experiments as described in Mair et al. (2019) . Proximity labelling of protein complexes and cell-type-specific organellar proteomes in Arabidopsis enabled by TurboID. Elife . 2019 Sep 19;8:e47864. doi: 10.7554/eLife.47864. This tag has MW of 35 kDa.

Reconstitution: N/A

Storage: Store at 4°C for 12-18 months. A preservative may be added for long time storage up to 2 years.

TAIR Nnumbre: TAIR

Category: GAL4 / GUS / LUC / others

Research Area: Plant Biology