Biotinylated Glucose AGE-BSA-II | 7930

Advanced glycation end products (AGEs) accumulate with age and at an accelerated rate in diabetes. AGEs bind cell-surface receptors including the receptor for AGE (RAGE). AGEs therefore have been implicated in the pathogenesis of diabetes, induction of proinflammatory cytokines, and stimulation of smooth muscle proliferation, and fibronectin production. The Biotinylated Glucose AGE-BSA-II was produced by biotinylation of Glucose AGE-BSA-II (Cat. # 2223-10) with Sulfo-NHS-LC-Biotin (Cat # 2326). Prior to biotinylation, The Glucose AGE BSA II was produced by reacting BSA with glucose under sterile conditions followed by extensive dialysis and purification steps. Fluorescence of AGEs was confirmed by fluorescence spectrophotometry with Ex/Em = 370/440 nm. Glycated BSA shows >50X increase in fluorescence in compared to control BSA.

7930 | Biotinylated Glucose AGE-BSA-II DataSheet

Sort Name: Biotinylated Glucose AGE-BSA-II

Label Name: Biotinylated Glucose AGE-BSA-II

Taglines: AGEs can bind a variety of receptors. Biotinylation of Glucose-AGE-BSA allows for its purification by Streptavidin-Sepharose beads

Product Highlights: FORM: Liquid. FORMULATION: 5 mg/ml of 0.22 µm filter sterilized solution in 1X PBS LABELING EFFICIENCY: ’s Biotinylated Glucose AGE-BSA-II was estimated to contain ~1-2 biotin/BSA (As tested using Biotin Quantitation Kit (K811-100)). APPLICATIONS: Biotinylation of Glucose AGE-BSA-II allows for its pull-down by Streptavidin-Sepharose beads (Cat. # 6565).

Packaging: EA