BiP | Lumenal-binding protein (rabbit antibody) | AS09 481

BiP | Lumenal-binding protein (rabbit antibody) | AS09 481 | Gentaur UK, US & Europe Distribution

Immunogen: KLH-conjugated synthetic peptide derived from Arabidopsis thaliana BiP proteins: BiP1 At5g28540 Q9LKR3, BiP2 At5g42020 F4K007, BiP3 At1g09080 Q8H1B3

Host: Rabbit

Conjugation: N/A

Clonality: Polyclonal

Isotype: N/A

Purity: Immunogen affinity purified serum in PBS pH 7.4.

Format: Lyophilized

Tested Application: ELISA (ELISA), Immunofluorescence (IF), Immunogold (IG), Western blot (WB)

Related Products: AS09 615 | Anti-BiP2 | lumenal-binding protein 2, goat antibodiesAS09 614 | Anti-BiP2 | lumenal-binding protein 2, chicken antibodiesAS09 481PRE | BiP | lumenal-binding protein, pre-immune serumantibodies to plant endomembrane system proteins Plant protein extraction bufferSecondary antibodies

Recommended Dilutions: 1 : 8000 (ELISA), 1 : 600 (IF), 1 : 2000 (WB)

Molecular weight: 73, 5 | 80 kDa

Confirmed Reactivity: Anacardium occidentale, Arabidopsis thaliana, Brassica napus, Chara australis R.Br, Chlamydomonas reinhardtii, Cucumis sativus, Mangifera indica, Moniliophthora perniciosa, Nicotiana benthamiana, Nicotiana tabacum, Raphanus sativa L. Tokinashi-daikon, Olea europaea, Oryza sativa, Picea abies, Pistachio sp., Physcomitrium patens, Schinus molle, Spinacia oleracea, Solanum lycopersicum, Solanum tuberosum, Triticum aestivum, Zea mays

Predicted Reactivity: Arabis alpina, Capsella rubella, Capsicum annuum, Citrus clementina, Citrus sinsensis, Eucalyptus grandis, Glycine max, Hordeum vulgare, Isatis tincorina, Prunus persica, Triticum aestivium, Petunia hybrida, Picea sitcHensis, Populus trichocarpa, Ricinus comminus, Vitis viniferaSpecies of your interest not listed? Contact us

Not reactive in: Ostreococcus tauri

Additional Information: Protein or membrane sample should be treated at 70°C for 10 min before loading on the gel, This antibody has so far not worked in IP

Background: BiP2 (Binding immunoglobulin protein) is localized in endoplasmic reticulum lumen (ER) and plays a role in protein assembly inside ER. BiP protein is abundant under all growth conditions but its synthesis can increase under conditions that lead to the accumulation of unfolded polypeptides in endoplasmic reticulum (ER) .

Reconstitution: For reconstitution add 50 µl of sterile water

Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

TAIR Nnumbre: AT5G42020, AT5G42020

Category: Endomembrane system

Research Area: Cell Biology