BLVRA, human recombinant | 7827

BLVRA, also known as biliverdin reductase A, belongs to the gfo/idh/mocA family. This protein is an enzyme that converts biliverdin to bilirubin, converting a double-bond between the second and third pyrrole ring into a single-bond. (Bilirubin + NAD (P) + = biliverdin + NAD (P) H) Recombinant BLVRA protein was expressed in E.coli and purified by using conventional chromatography techniques.

7827 | BLVRA human recombinant DataSheet

Biomolecule/Target: BLVRA

Synonyms: Biliverdin reductase A, BLVR, BVR, BVRA

Alternates names: Biliverdin reductase A, BLVR, BVR, BVRA

Taglines: An enzyme that converts biliverdin to bilirubin

NCBI Gene ID #: 1514

NCBI Gene Symbol: CTSL1

Gene Source: Human

Accession #: P07711

Recombinant: Yes

Source: E. coli.

Purity by SDS-PAGEs: 90%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: < 1.0 EU per 1 g of protein (determined by LAL method)

Activity (Specifications/test method): N/A

Biological activity: Specific activity is > 30 pmoles/min/µg. One unit BLVRA will transform 1.0 pmole of biliverdin to bilirubin per minute in an NADPH dependent reaction pH 8.0 at 25ºC.

Results: Specific activity is > 30 pmoles/min/µg.

Binding Capacity: N/A

Unit Definition: One unit BLVRA will transform 1.0 pmole of biliverdin to bilirubin per minute in an NADPH dependent reaction pH 8.0 at 25ºC.

Molecular Weight: 33.3 kDa (295 aa, 3-296 aa)

Concentration: 1 mg/ml

Appearance: Liquid

Physical form description: 1 mg/ml solution in 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Reconstitution Instructions: N/A

Amino acid sequence: MAEPERKFGV VVVGVGRAGS VRMRDLRNPH PSSAFLNLIG FVSRRELGSI DGVQQISLED ALSSQEVEVA YICSESSSHE DYIRQFLNAG KHVLVEYPMT LSLAAAQELW ELAEQKGKVL HEEHVELLME EFAFLKKEVV GKDLLKGSLL FTAGPLEEER FGFPAFSGIS RLTWLVSLFG ELSLVSATLE ERKEDQYMKM TVCLETEKKS PLSWIEEKGP GLKRNRYLSF HFKSGSLENV PNVGVNKNIF LKDQNIFVQK LLGQFSEKEL AAEKKRILHC LGLAEEIQKY CCSRK