BORA Polyclonal Antibody | E-AB-53604

BORA Polyclonal Antibody | E-AB-53604 | Gentaur UK, US & Europe Distribution

Type: Polyclonal Antibody

Synonyms: Aurora borealis, bora, bora, aurora kinase A activator, BORA, C13orf34, Chromosome 13 open reading frame 34 , FLJ22624, HsBora, Protein aurora borealis, RP11-342J4.2

Application: WB, IHC, ELISA

Reactivity: Human

Host: Rabbit

Isotype: IgG

Reserch Areas: Cancer, Cell Biology, Signal transduction

Background: Aurora related kinase-1 (ARK-1) is a centrosome-associated serine/threonine kinase that regulates centrosome separation, bipolar spindle assembly and chromosome segregation during mitosis. Bora (protein aurora borealis) is a 559 amino acid protein that activates ARK-1. Bora is localized to the nucleus until mitosis is initiated, when it then translocates to the cytoplasm. This translocation is dependent on activated Cdc2, which releases Bora to bind and activate ARK-1 in the cytoplasm. Plk (polo-like kinase) interacts with Bora to control the accessibility of its activation loop for phosphorylation and activation on its N-terminus by ARK-1. It is through this mechanism that Bora and ARK-1 control cellular mitotic entry. Downregulation of the gene encoding Bora results in multipolar spindles in mitosis, a phenomenon that is also observed when ARK-1 function is blocked.

Concentration: 1.8 mg/mL

Storage: Store at -20°C. Avoid freeze / thaw cycles.

Immunogen: Synthetic peptide of human BORA

Buffer: PBS with 0.05% NaN3 and 40% Glycerol, pH7.4

Purification Method: Antigen affinity purification

Dilution: WB 1:500-1:2000, IHC 1:50-1:300, ELISA 1:5000-1:10000

Clone: N/A

Conjugation: Unconjugated

Molecular Weight(Calculated): 61 kDa

Molecular Weight(Observed): Refer to figures