Bovine RELT-like protein 1 (RELL1) ELISA Kit | AE59913BO

Bovine RELT-like protein 1 (RELL1) ELISA Kit | AE59913BO | Gentaur UK, US & Europe Distribution

Species Reactivity: Bovine (Bos taurus; Cattle)

Abbreviation: RELL1

Alternative Name: FLJ21778; MGC50583; receptor expressed in lymphoid tissues like 1|tmp_locus_29

Application: ELISA

Range: 31.25-2000 pg/mL

Sensitivity: 13.4 pg/mL

Intra-Assay: ≤4.7%

Inter-Assay: ≤8.3%

Recovery: 0, 96

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate RELL1 in samples. An antibody specific for RELL1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyRELL1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for RELL1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of RELL1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: The predicted RELL1 sequence contains a transmembrane domain but lacks extracellular cysteine-rich domains. RELL1 shares 32% and 40% amino acid identity with RELT and RELL2, respectively. Northern blot analysis detected an approximately 4-kb transcript in all human tissues examined with highest expression in placenta, spleen, skeletal muscle, and testis. Immunofluorescent confocal microscopy localized RELL1 to the plasma membrane in COS-7 cells and colocalized RELL1 with RELL2 and RELT. Coimmunoprecipitation studies showed that RELL1 interacted with RELL2, RELT, and OSR1. By in vitro kinase assay, Cusick et al. (2006) showed that RELL1, RELL2, and RELT were phosphorylated by OSR1.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .