Bovine Speckle-type POZ protein (SPOP) ELISA Kit | AE15014BO

Bovine Speckle-type POZ protein (SPOP) ELISA Kit | AE15014BO | Gentaur UK, US & Europe Distribution

Species Reactivity: Bovine (Bos taurus; Cattle)

Abbreviation: SPOP

Alternative Name: TEF2;

Application: ELISA

Range: Request Information

Sensitivity: Request Information

Intra-Assay: ≤6.3%

Inter-Assay: ≤10.1%

Recovery: 1, 05

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate SPOP in samples. An antibody specific for SPOP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySPOP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SPOP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPOP bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: SPOP encodes a protein that may modulate the transcriptional repression activities of death-associated protein 6 (DAXX), which interacts with histone deacetylase, core histones, and other histone-associated proteins. In mouse, the encoded protein binds to the putative leucine zipper domain of macroH2A1.2, a variant H2A histone that is enriched on inactivated X chromosomes. The BTB/POZ domain of this protein has been shown in other proteins to mediate transcriptional repression and to interact with components of histone deacetylase co-repressor complexes. Alternative splicing of this gene results in multiple transcript variants encoding the same protein.The SPOP gene encodes a 374-amino acid polypeptide that contains a poxvirus and zinc finger (POZ) domain and an evolutionarily conserved N-terminal region.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .