Bovine Transmembrane protein 66 (TMEM66) ELISA Kit | AE14239BO

Bovine Transmembrane protein 66 (TMEM66) ELISA Kit | AE14239BO | Gentaur UK, US & Europe Distribution

Species Reactivity: Bovine (Bos taurus; Cattle)

Abbreviation: TMEM66

Alternative Name: FLJ22274; FOAP-7; HSPC035; MGC8721; XTP3;

Application: ELISA

Range: Request Information

Sensitivity: Request Information

Intra-Assay: ≤4.1%

Inter-Assay: ≤8.3%

Recovery: 1, 02

Sample Type: Serum, Plasma, Other biological fluids

Detection Method: Sandwich

Analysis Method : Quantitive

Test Principale: This assay employs a two-site sandwich ELISA to quantitate TMEM66 in samples. An antibody specific for TMEM66 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyTMEM66 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMEM66 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMEM66 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Product Overview: Transmembrane protein 66 play important roles in cell apoptosis and tumorigenesis. In order to investigate the function of TMEM66 gene, the transgenic mouse model containing the mutant TMEM66 gene was constructed in this study. The transgenic mice were generated by u-sing microinjection method and 312 injected zygotes were implanted into 13 foster mothers. The positive transgenic mice were confirmed by PCR and Southern blotting. Also, the stability of the transgene was confirmed by passage and the way of integration of the transgene was studied using inverse PCR method. In-verse PCR results indicated that the transgenic DNA fragment was integrated into the host ge-nome in a serial multi-copy manner. Therefore, the transgenic mice model containing the mutant TMEM66 gene was constructed successfully.

Stability: The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C) .