BRCA2 Antibody [AMM02547G]

BRCA2 Antibody [AMM02547G] | Gentaur UK, US & Europe Distribution

Product Category: Monoclonal Antibodies

Host: Mouse

Species Reactivity: H

Specificity: This BRCA2 antibody is generated from a mouse immunized with a recombinant protein between 251-495 amino acids from human BRCA2.

Cellular Localisation: Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Note=Colocalizes with ERCC5/XPG to nuclear foci following DNA replication stress

Molecular Weight: 384202

Clone: Monoclonal

Gene Name: BRCA2 (HGNC:1101)

Gene ID: 675

Function: Involved in double-strand break repair and/or homologous recombination. Binds RAD51 and potentiates recombinational DNA repair by promoting assembly of RAD51 onto single-stranded DNA (ssDNA) . Acts by targeting RAD51 to ssDNA over double-stranded DNA, enabling RAD51 to displace replication protein-A (RPA) from ssDNA and stabilizing RAD51- ssDNA filaments by blocking ATP hydrolysis. Part of a PALB2-scaffolded HR complex containing RAD51C and which is thought to play a role in DNA repair by HR. May participate in S phase checkpoint activation. Binds selectively to ssDNA, and to ssDNA in tailed duplexes and replication fork structures. May play a role in the extension step after strand invasion at replication-dependent DNA double-strand breaks; together with PALB2 is involved in both POLH localization at collapsed replication forks and DNA polymerization activity. In concert with NPM1, regulates centrosome duplication. Interacts with the TREX-2 complex (transcription and export complex 2) subunits PCID2 and SEM1, and is required to prevent R-loop-associated DNA damage and thus transcription-associated genomic instability. Silencing of BRCA2 promotes R-loop accumulation at actively transcribed genes in replicating and non-replicating cells, suggesting that BRCA2 mediates the control of R-loop associated genomic instability, independently of its known role in homologous recombination (PubMed:24896180) .

Summary: Tissue Location: Highest levels of expression in breast and thymus, with slightly lower levels in lung, ovary and spleen

Form: Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein G column, followed by dialysis against PBS.

Storage: Store at +4°C short term. For long-term storage, aliquot and store at -20°C or below. Stable for 12 months at -20°C. Avoid repeated freeze-thaw cycles.

Application: WB

Dilution: WB--1:2000

Synonyms: Breast cancer type 2 susceptibility protein, Fanconi anemia group D1 protein, BRCA2, FACD, FANCD1