BRD4 Antibody | 6639

The acetylation of histone lysine residues plays a crucial role in the epigenetic regulation of gene transcription. A bromodomain is a protein domain that recognizes acetylated lysine residues such as those on the N-terminal tails of histones. This recognition is often a prerequisite for protein-histone association and chromatin remodeling. These domains function in the linking of protein complexes to acetylated nucleosomes, thereby controlling chromatin structure and gene expression. Thus, bromodomains serve as “readers” of histone acetylation marks regulating the transcription of target promoters. The BET family of proteins, defined by tandem Bromodomains and an Extra Terminal domain, include BRD2, BRD3, BRD4, and BRDT. The BET proteins play a key role in many cellular processes, including inflammatory gene expression, mitosis, and viral/host interactions. The isolated individual or tandem bromodomains of BRD2 and BRD4 have been shown to bind acetylated histone tails, serving to couple histone acetylation marks to the transcriptional regulation of target promoters. Small molecule inhibitors of these interactions hold promise as useful therapeutics for human disease.

6639 | BRD4 Antibody DataSheet

Antibody Target: BRD4

Target Alternative Name: BRD4, Protein HUNK1, HUNK1

Tag Line: Polyclonal Antibody to detect BRD4 in human, mouse and rat samples

Category: BRD4

Host: Rabbit

Isotype: Rabbit IgG

Species Reactivities: Human, mouse, rat

Immunogen Sequence: Recombinant protein

Accession #: O60885

Gene ID: 23476

Appearance: Colorless liquid

Form: Liquid

Concentration: 0.5 mg/ml

Formulation: In phosphate buffered saline (PBS), pH 7.2, containing 30% glycerol, 0.5% BSA, 5 mM EDTA and 0.03% proclin.

Purification: Affinity Purified Antibody

Application: Western blot

Positive Control: Western Blot: Recombinant protein

Application And Usages: Western blot: 1:200

Country of Animal Origin: USA

Country of Manufacture: USA

Usage: For Research Use Only! Not to be used in humans.

Handling: The antibody solution should be gently mixed before use.

Western Blot Verified: Verified

Immunocytochemistry Verified: Not Verified

Immunofluorescence Verified: Not Verified

Immunoprecipitation Verified: Not Verified

FACS Verified:

ELISA Verified: Not Verified

ChIP Verified: Not Verified

Dot Blot Verified:

Flow Cytometry Verified: Not Verified