Bru-Com RT-PCR | TVET9-50FRT

Bru-Com RT-PCR | TVET9-50FRT is available for delivery

Real Time Kit for use with Rotor-Gene™ 2000/3000/6000 (Corbett Research, Qiagen)

Storage & Shipping :

Stable for up to one year when stored at –20°C, store it at –70°C or below for longer periods.

Bru-Com RT-PCR TVET9-50FRT DataSheet

INTENDED USE

The Bru-Com Real-TM is a “Real-Time Amplification” test for the qualitative detection of Brucella species (B.melitensis, B.abortus, B.suis, B.ovis, B.canis) in the biological materials (whole blood, milk, tissue,etc) in the Rotor-Gene™ 2000/3000/6000/Q. Test contains an Internal Control (IC) which serves as an amplification control for each individually processed specimen and to identify possible reaction inhibition.

PERFORMANCE CHARACTERISTICS

Analytical specificity

The analytical specificity of the primers and probes was validated with negative samples. They did not generate any signal with the specific Brucella primers and probes. The specificity of the kit Bru-Com Real-TM was 100%. The potential cross-reactivity of the kit Bru-Com Real-TM was tested against the group control. It was not observed any cross-reactivity with other pathogens.

Analytical sensitivity

The kit Bru-Com Real-TM allows to detect Brucella DNA in 100% of the tests with a sensitivity of not less than 1000 copies/ml. The detection was carried out on the control standard and its dilutions by negative sample.