BRUCE Antibody | 3309

BRUCE Antibody | 3309 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human

Homology: Predicted species reactivity based on immunogen sequence: Mouse: (100%)

Immunogen: BRUCE antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human BRUCE.
The immunogen is located within amino acids 4760 - 4810 of BRUCE.

Research Area: Apoptosis

Tested Application: E, IHC-P

Application: BRUCE antibody can be used for detection of BRUCE by immunohistochemistry at 5 μg/mL.
Antibody validated: Immunohistochemistry in human samples. All other applications and species not yet tested.

Specificiy: BRUCE antibody is human specific. Multiple isoforms of BRUCE are known to exist.

Positive Control 1: Cat. No. 11-801 - Human Small Intestine Tissue Slide

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: 534 kDa

Validation: N/A

Isoform: N/A

Purification: BRUCE Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: BRUCE Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: BRUCE antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: BRUCE Antibody: BRUCE, APOLLON, KIAA1289, Baculoviral IAP repeat-containing protein 6, BIR repeat-containing ubiquitin-conjugating enzyme, BRUCE

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: BRUCE Antibody: Apoptosis, or programmed cell death, is related to many diseases, such as cancer. Apoptosis is triggered by a variety of stimuli including members in the TNF family and can be prevented by the inhibitor of apoptosis (IAP) proteins. IAP proteins form a conserved gene family that binds to and inhibits cell death proteases. BRUCE, also known as BIRC6, is an IAP family member protein with a BIR (baculoviral inhibition of apoptosis protein repeat) domain and a UBCc (ubiquitin-conjugating enzyme E2, catalytic) domain. BRUCE regulates p53 and the mitochondrial pathway of apoptosis by facilitating the degradation of apoptotic proteins such as Caspase-9 and SMAC by ubiquitination.