BRUCELLA ABORTUS ANTIGEN

Brucella abortus antigen is a native lysate, manufactured by fermentation for use in immunoassay development and other research applications.

PRODUCT DETAILS – BRUCELLA ABORTUS ANTIGEN

• Brucella abortus lysate, produced by fermentation.
• Strain – S99 Weybridge
• Presented in glycine buffer (pH 9.5) containing n-octyl-β-D-glucopyranoside.
• For immunoassay development or other applications.

BACKGROUND

Brucellosis is a zoonotic disease in cattle, mainly caused by B. abortus. It is estimated that more than 300 million of the 1.4 billion worldwide cattle population is infected with the pathogen (de Figueiredo et al., 2015). Infection in dairy herds causes abortion, stillbirth, postpartum metritis, and inflammation of the mammary gland, leading to decreased milk production and shedding of the organism in the milk of infected cows. Last-trimester abortion is the predominant clinical sign of bovine brucellosis, but infected cows usually present with only one episode of abortion, giving birth to weak or even healthy calves in subsequent gestations. Many infected cows remain asymptomatic throughout their lifetime, giving birth to healthy calves, while still shedding the organism in the placenta. In bulls, B. abortus is a common cause of temporary or permanent infertility, leading to orchitis, epididymitis, and seminal vesiculitis. The disease is transmitted by contact between susceptible individuals and the contaminated fetus, fetal membranes, and uterine secretions from an infected cow (reviewed in Byndloss & Tsoli, 2016). Although different vaccines are available they have many drawbacks including residual virulence, serodiagnostic interference and low levels of protection (Lalsiamthara & Lee, 2017; Khan & Zahoor, 2018).

REFERENCES

• Byndloss MX, Tsolis RM. Brucella spp. Virulence Factors and Immunity. Annu Rev Anim Biosci. 2016;4:111–127.
• de Figueiredo P, Ficht TA, Rice-Ficht A, Rossetti CA, Adams LG. Pathogenesis and immunobiology of brucellosis: review of Brucella-host interactions. Am J Pathol. 2015;185(6):1505‐1517.
• Khan MZ, Zahoor M. An Overview of Brucellosis in Cattle and Humans, and its Serological and Molecular Diagnosis in Control Strategies. Trop Med Infect Dis. 2018;3(2):65.
• Lalsiamthara J, Lee JH. Development and trial of vaccines against Brucella. J Vet Sci. 2017;18(S1):281‐290.