Bu-1 Antibody [AV20] | 99-310

Bu-1 Antibody [AV20] | 99-310 | Gentaur UK, US & Europe Distribution

Host: Mouse

Reactivity: Chicken

Homology: N/A

Immunogen: Chicken peripheral blood lymphocytes and splenocytes

Research Area: Other

Tested Application: WB, IHC-Fr, ICC, Flow, IP

Application: Bu-1 Antibody [AV20] for use in flow cytometry, immunohistochemistry / immunocytochemistry, and immunoprecipitation assays.

Specificiy: Bu-1

Positive Control 1: N/A

Positive Control 2: N/A

Positive Control 3: N/A

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: N/A

Clonality: Monoclonal

Clone: [AV20]

Isotype: IgG1

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Supplied in borate buffered saline, pH 8.2; No preservatives or amine-containing buffer salts added

Concentration: 0.5 mg/mL

Storage Condition: Store vial at 2-8˚C

Alternate Name: ChB6

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: Chicken Bu-1, a product of the Bu-1a and Bu-1b alleles, is a type I transmembrane glycosylated disulfide-linked homodimer. The monoclonal antibody AV20 recognizes a monomorphic determinant on the Bu-1 B cell-associated alloantigens of both RPL 6 (3) (Bu-1a) and 7 (2) (Bu-1b) lines of inbred chickens. The antibody also identifies B cells in partially inbred birds. Bu-1 is found on 85-90% of bursal cells, 2-8% of thymocytes, 15-27% of spleen cells, and 2-18% of peripheral blood cells. It is also expressed on subsets of macrophages and monocytes but not on granulocytes, erythrocytes, or thrombocytes. The monoclonal antibody AV20 does not cross react with turkey cells when analyzed by flow cytometry.