Calmodulin, Bovine Brain | 7291

Calmodulin (CaM) is a ubiquitous, calcium-binding protein that can bind to and regulate a multitude of different protein targets, thereby affecting many different cellular functions. CaM mediates processes such as inflammation, metabolism, apoptosis, muscle contraction, intracellular movement, short-term and long-term memory, nerve growth and the immune response. CaM is expressed in many cell types and can have different subcellular locations, including the cytoplasm, within organelles, or associated with the plasma or organelle membranes. Many of the proteins that CaM binds are unable to bind calcium themselves, and as such use CaM as a calcium sensor and signal transducer. CaM can also make use of the calcium stores in the endoplasmic reticulum, and the sarcoplasmic reticulum. CaM undergoes a conformational change upon binding to calcium, which enables it to bind to specific proteins for a specific response. CaM can bind up to four calcium ions, and can undergo post-translational modifications, such as phosphorylation, acetylation, methylation and proteolytic cleavage, each of which can potentially modulate its actions.

7291 | Calmodulin Bovine Brain DataSheet

Biomolecule/Target: Calmodulin

Synonyms: CAM, CALM

Alternates names: Fibroblast growth factor 19

Taglines: Binds to and regulates a multitude of different protein targets

NCBI Gene ID #: 9965

NCBI Gene Symbol: FGF19

Gene Source: Bovine

Accession #: NM_005117

Recombinant: No

Source: Bovine Brain

Purity by SDS-PAGEs: 95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): N/A

Biological activity: Determined by a cell proliferation assay using balb/c 3T3 cells. The expected ED for this effect is 50-200 ng/ml.

Results: 2.5 units per mg protein. One unit is defined as the amount of enzyme that hydrolyzes one µmole of N-benzoyl-DL-arginine-pNA per minute at 25°C in 200 mM Tris-HCl, pH 7.8, with 20 mM CaCl2.

Binding Capacity: N/A

Unit Definition: One unit is defined as the amount of enzyme that hydrolyzes one µmole of N-benzoyl-DL-arginine-pNA per minute at 25°C in 200 mM Tris-HCl, pH 7.8, with 20 mM CaCl2.

Molecular Weight: 21.8 kDa

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized in 30 mM Hepes, pH 7.4, 1 mM CaCl2 and 0.1 mM DTT.

Reconstitution Instructions: Reconstitute in HO to a concentration of 0.1 to 1.0 µg/µl. This solution can then be diluted into other aqueous buffers

Amino acid sequence: N/A