Carabin Antibody | 4267

Carabin Antibody | 4267 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse

Homology: N/A

Immunogen: Carabin antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human Carabin.
The immunogen is located within the last 50 amino acids of Carabin.

Research Area: Immunology

Tested Application: E, WB, IHC-P

Application: Carabin antibody can be used for detection of Carabin by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested.

Specificiy: N/A

Positive Control 1: Cat. No. 1224 - Daudi Cell Lysate

Positive Control 2: Cat. No. 1306 - Human Spleen Tissue Lysate

Positive Control 3: Cat. No. 10-901 - Human Spleen Tissue Slide

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: N/A

Validation: N/A

Isoform: N/A

Purification: Carabin Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Carabin Antibody is supplied in PBS containing 0.02% sodium azide.

Concentration: 1 mg/mL

Storage Condition: Carabin antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: Carabin Antibody: EPI64C, CARABIN, Carabin, TBC1 domain family member 10C

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: Carabin Antibody: Antigen binding by the T-cell receptor (TCR) is one of the critical first steps in the immune response, triggering a cascade of signaling pathways that ultimately lead to T-cell activation. Screening a yeast two-hybrid screen of a human T-cell cDNA library with calcineurin, a protein phosphatase involved in multiple signaling pathways including T-cell activation, resulted in the identification of Carabin, a member of the TBC1 domain family of proteins, as a calcineurin-binding protein. Unlike other members of the TBC1 domain protein family which are thought to have a role in regulating cell growth and differentiation, further experiments demonstrated that Carabin is part of a negative regulatory loop for the intracellular TCR signaling pathway as well as an inhibitor of the Ras signaling pathway, suggesting that Carabin may also mediate crosstalk between calcineurin and Ras. Carabin antibody does not recognize TBC1D10A or TBC1D10B. Carabin is known to exist in multiple isoforms.