Carbonic anhydrase-1, human recombinant | P1048

Carbonic anhydrase (CA) is an enzyme that catalyses rapid conversion of carbon dioxide to bicarbonate and protons (CO2 + H2O HCO3 + H+). Most carbonic anhydrases contain a zinc ion in their active site and the primary function of this enzyme is known to maintain acid-base balance in blood and other tissues, and to help transport carbon dioxide of tissues. Carbonic anhydrases have been found in all kingdoms of life. Recombinant carbonic anhydrase fused to His-tag, was expressed in E.coli and purified by conventional chromatography techniques. Specific activity is >450 pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0 pmole of 4-nitrophenyl acetate to 4-nitrophenol per minute at pH 7.5 at 37C.

P1048 | Carbonic anhydrase-1 human recombinant DataSheet

Biomolecule/Target: CA1

Synonyms: CA1, CA-I, CAB

Alternates names: Melanoma differentiation association like protein, MDA1, NG.1, ZMDA1, IL-10C, IL-19

Taglines: Catalyzes the reversible hydration of carbon dioxide in various biological processes.

NCBI Gene ID #: 29949

NCBI Gene Symbol: IL19

Gene Source: Human

Accession #: Q9UHD0

Recombinant: Yes

Source: E. coli

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): Measured by its ability to hydrolyze 1.0 pmole of 4-nitrophenyl acetate to 4-nitrophenol per minute at pH 8 at 37C.

Biological activity: Test in process

Results: >300 pmol/min/ug

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 17.9 kDa

Concentration: N/A

Appearance: Liquid

Physical form description: In 20 mM Tris-HCl buffer (pH8.0) containing 1mM DTT, 10% glycerol

Reconstitution Instructions: Reconstitute in HO to a concentration of 0.1 to 1.0 g/l. It is recommended that further dilutions be made into buffer containing carrier protein or medium containing serum.

Amino acid sequence: N/A