Carbonic anhydrase-8, human recombinant | P1047

CA8 was initially named CA-related protein because of sequence similarity to other known carbonic anhydrase genes. However, this protein lacks carbonic anhydrase activity (i.e., the reversible hydration of carbon dioxide). It continues to carry a carbonic anhydrase designation based on clear sequence identity to other members of the carbonic anhydrase gene family. Defects in CA8 are the cause of cerebellar ataxia mental retardation and dysequilibrium syndrome type 3 (CMARQ3). Recombinant human CA8 protein fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

P1047 | Carbonic anhydrase-8 human recombinant DataSheet

Biomolecule/Target: CA8

Synonyms: CA-VIII, CALS, CAMRQ3, CARP, CA8

Alternates names: Melanoma differentiation association like protein, MDA1, NG.1, ZMDA1, IL-10C, IL-19

Taglines: A protein lacks carbonic anhydrase activity, involved in Cerebellar ataxia, mental retardation, and dysequilibrium syndrome 3 (CMARQ3).

NCBI Gene ID #: 29949

NCBI Gene Symbol: IL19

Gene Source: Human

Accession #: Q9UHD0

Recombinant: Yes

Source: E. coli

Purity by SDS-PAGEs: 98%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: N/A

Activity (Specifications/test method): Measured by its ability to hydrolyze 1.0 pmole of 4-nitrophenyl acetate to 4-nitrophenol per minute at pH 7.5 at 37C.

Biological activity: Test in process

Results: >450 pmol/min/ug

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 17.9 kDa

Concentration: N/A

Appearance: Liquid

Physical form description: In 20 mM Tris-HCl buffer (pH8.0) containing 1mM DTT, 20% glycerol

Reconstitution Instructions: Reconstitute in HO to a concentration of 0.1 to 1.0 g/l. It is recommended that further dilutions be made into buffer containing carrier protein or medium containing serum.

Amino acid sequence: N/A