Carbonic Anhydrase 3, human recombinant | 7833

Carbonic anhydrase 3 (CA3) is also known as Carbonate dehydratase III, Carbonic anhydrase III, which belongs to the alpha-carbonic anhydrase family. These carbonic anhydrases are a class of metalloenzymes that catalyze the reversible hydration of carbon dioxide and are differentially expressed in a number of cell types. The catalytic activity of carbonic anhydrase 3 is activated by proton donors such as imidazole and the dipeptide histidylhistidine and is inhibited by coumarins and sulfonamide derivatives such as acetazolamide.

7833 | Carbonic Anhydrase 3 human recombinant DataSheet

Biomolecule/Target: Carbonic Anhydrase 3

Synonyms: CA3, Carbonic anhydrase 3

Alternates names: Fibroblast growth factor 19

Taglines: Catalyzes the reversible hydration of carbon dioxide

NCBI Gene ID #: 9965

NCBI Gene Symbol: FGF19

Gene Source: Human

Accession #: NM_005117

Recombinant: Yes

Source: E. coli.

Purity by SDS-PAGEs: 95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: N/A

Endotoxin Level: <1 EU/g by LAL method

Activity (Specifications/test method): Measured by its esterase activity. The specific activity is >1 pmol/min/µg.

Biological activity: Determined by a cell proliferation assay using balb/c 3T3 cells. The expected ED for this effect is 50-200 ng/ml.

Results: The specific activity is >1 pmol/min/µg.

Binding Capacity: N/A

Unit Definition: N/A

Molecular Weight: 21.8 kDa

Concentration: N/A

Appearance: Lyophilized

Physical form description: Lyophilized from 0.22 m filtered solution in 50 mM Tris, 150 mM NaCl, pH 7.5. Normally Mannitol or Trehalose are added as protectants before lyophilization.

Reconstitution Instructions: Reconstitute in HO to a concentration of 0.1 to 1.0 µg/µl. This solution can then be diluted into other aqueous buffers

Amino acid sequence: N/A