Cas9 | CRISPR-associated endonuclease 9 (polyclonal) | AS16 3690

Cas9 | CRISPR-associated endonuclease 9 (polyclonal) | AS16 3690 | Gentaur UK, US & Europe Distribution

Immunogen: KLH-conjugated synthetic peptide derived from Streptococcus thermophilus Cas9/Csn1 protein sequence, UniProt: Q03JI6 (peptide sequence is conserved in pYLCRISPR/Cas9Pubi-H vector)

Host: Rabbit

Conjugation: N/A

Clonality: Polyclonal

Isotype: N/A

Purity: Immunogen affinity purified serum in PBS pH 7.4.

Format: Lyophilized

Tested Application: Immunolocalization (IL), Western blot (WB)

Related Products: AS16 3841 | Anti/Cpf1 | CRISPR from Prevotella and Francisella 1, rabbit antibodiesCollection of antibodies to tag proteinsPlant and algal protein extraction buffer Secondary antibodies

Recommended Dilutions: 1 : 100 (IL), 1 : 1000 (WB)

Molecular weight: Depends upon a MW of a protein which is a fusion partner,

Confirmed Reactivity: Staphylococcus aureus

Predicted Reactivity: Staphylococcus pyogenes UniProt: Q99ZW2

Not reactive in: N/A

Additional Information: N/A

Background: Cas9 (CRISPR-associated endonuclease 9) is an RNA-guided DNA endonuclease associated with the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) type II adaptive immunity system. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) . Cas9 protein serves as a genome engineering tool to induce site-directed double strand breaks in DNA.

Reconstitution: For reconstitution add 50 µl of sterile water

Storage: Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

TAIR Nnumbre: N/A

Category: CRISPR

Research Area: Gene Editing/CRISPR