Caspase-1 Substrate YVAD-AFC | 1103

Ready-to-use fluorometric substrate for caspase-1/ICE and related caspases that recognize the amino acid sequence YVAD. Caspase-1 and related caspase activity can be quantified by fluorescent detection of free AFC after cleaved from the peptide substrate YVAD-AFC at Ex. = 400 nm and Em. = 505 nm, using a fluorometer or multi-well fluorescence plate reader. Alternatively, a shift in fluorescence from blue to green upon cleavage can be visualized, using a hand-held long-UV lamp. The ready-to-use caspase substrate provides an economic alternative for researchers who perform large volume caspase assays. Cell Lysis Buffer (Cat. #1067-100, -400) and 2X Reaction Buffer (Cat. #1068-20, -80), and DTT used for caspase assays are also available separately.

YVAD-AFC is a ready-to-use fluorometric substrate for caspase-1/ICE and related caspases that recognize the amino acid sequence YVAD.

1103 | Caspase-1 Substrate YVAD-AFC DataSheet

Alternate Name/Synonyms: Ac-YVAD-AFC, Caspase-1 Substrate, Fluorogenic

Appearance: Liquid

Formulation: DMSO ( 1 mM)

CAS Number: N/A

Structure Available?: No

Peptide sequence: Ac-Tyr-Val-Ala-Asp-AFC

Salt Form: No

Molecular Formula: C₃₃H₃₆F₃N₅O₁₀

Molecular Weight: 719.7

Cell-Permeable?: No

Purity: >99% by HPLC

Solubilities: N/A

Handling: Protect from light and moisture

Country of Origin: USA

Tag Line: A caspase-1 substrate

MDL Number: MFCD01318881

PubChem CID: 44135090

SMILES: CC(C)C(C(=O)NC(C)C(=O)NC(CC(=O)O)C(=O)NC1=CC2=C(C=C1)C(=CC(=O)O2)C(F)(F)F)NC(=O)C(CC3=CC=C(C=C3)O)NC(=O)C

InChi: InChI=1S/C33H36F3N5O10/c1-15(2)28(41-31(49)23(38-17(4)42)11-18-5-8-20(43)9-6-18)32(50)37-16(3)29(47)40-24(14-26(44)45)30(48)39-19-7-10-21-22(33(34,35)36)13-27(46)51-25(21)12-19/h5-10,12-13,15-16,23-24,28,43H,11,14H2,1-4H3,(H,37,50)(H,38,42)(H,39,48)(H,40,47)(H,41,49)(H,44,45)

InChi Key: GWKJISJKNKIJNP-UHFFFAOYSA-N