Caspase-10/a, human recombinant | 1090A

Caspase-10/a (also known as Mch4) is a member of the caspase-family of cysteine proteases. Similar to other caspases, caspase-10 also exists in cells as an inactive proenzyme. During apoptosis procaspase-10 is processed at aspartate residues to form active caspase-10 which consists of two large (18 kDa) and two small (11 kDa) subunits in a heterotetramer form. The recombinant active human caspase-10 was expressed in E. coli. The active caspase-10 is routinely tested at for its ability to enzymatically cleave these two substrates Ac-IETD-pNA (Cat. #1063-200) or Ac-IETD-AFC (1062-200).

1090A | Caspase-10/a human recombinant DataSheet

Biomolecule/Target: Caspase-10/a

Synonyms: Caspase-10/a

Alternates names: IL10, D ZCYTO10, IL-20, MGC96907, Four helix cytokine, Zcyto10,

Taglines: A cysteine protease involved in apoptosis

NCBI Gene ID #: 58181

NCBI Gene Symbol: IL20

Gene Source: Human

Accession #: Q9JKV9

Recombinant: Yes

Source: E. coli.

Purity by SDS-PAGEs: 95%

Assay: SDS-PAGE

Purity: N/A

Assay #2: HPLC

Endotoxin Level: N/A

Activity (Specifications/test method): > 8000 units/mg

Biological activity: Mouse IL-20 is fully biologically active when compared to standard. The biological activity was determined by measuring the dose-dependent activation of STAT3 in human epidermal keratinocytes (HEK2a cells). Significant STAT3 activation is observed with >100 ng/ml of recombinant mouse IL-20

Results: N/A

Binding Capacity: N/A

Unit Definition: One unit of the recombinant caspase-10/a is the enzyme activity that cleaves 1 nmol of the caspase substrate IETD-pNA (pNA: pnitroanaline) per hour at 37°C in a reaction solution containing 50 mM Hepes, pH 7.2, 50 mM NaCl, 0.1% Chaps, 10 mM EDTA, 5% Glycerol, and 10 mM DTT.

Molecular Weight: 17.6 kDa

Concentration: N/A

Appearance: Lyophilized powder

Physical form description: Lyophilized powder

Reconstitution Instructions: Reconstitute in HO to a concentration of 0.1-0.5 mg/ml (Note: Gently pipetting to mix). The solution can be diluted into other aqueous buffers and stored at 4°C for 1 week or –20°C for future use.

Amino acid sequence: N/A