Caspase-10 Substrate AEVD-AFC | 1113

Ready-to-use fluorometric substrate for AEVD-dependent caspases. Activity of AEVD-dependent caspase activity can be quantified by fluorescent detection of free AFC after cleavage from the peptide substrate AEVD-AFC at Ex. = 400 nm and Em. = 505 nm, using a fluorometer or multi-well fluorescence plate reader. Alternatively, a shift in fluorescence from blue to green upon cleavage can be visualized using a hand-held long-UV lamp. Cell Lysis Buffer (Cat. #1067-100, -400), 2X Reaction Buffer (Cat. #1068-20, -80), DTT (1201-1) and other reagents used for caspase assays are also available separately.

AEVD-AFC is a ready-to-use fluorometric substrate for caspase-10 and related caspases that recognize the amino acid sequence AEVD.

1113 | Caspase-10 Substrate AEVD-AFC DataSheet

Alternate Name/Synonyms: Ac-AEVD-AFC, Caspase-10 Substrate, Fluorogenic

Appearance: Liquid

Formulation: DMSO (1 mM)

CAS Number: N/A

Structure Available?: No

Peptide sequence: Ac-Ala-Glu-Val-Asp-AFC

Salt Form: No

Molecular Formula: C₂₉H₃₄F₃N₅O₁₁

Molecular Weight: 685.6

Cell-Permeable?: No

Purity: >98% by HPLC

Solubilities: N/A

Handling: Protect from light and moisture

Country of Origin: USA

Tag Line: A caspase-10 substrate

MDL Number: N/A

PubChem CID: 44134611

SMILES: CC(C)C(C(=O)NC(CC(=O)O)C(=O)NC1=CC2=C(C=C1)C(=CC(=O)O2)C(F)(F)F)NC(=O)C(CCC(=O)O)NC(=O)C(C)NC(=O)C

InChi: InChI=1S/C29H34F3N5O11/c1-12(2)24(37-26(45)18(7-8-21(39)40)35-25(44)13(3)33-14(4)38)28(47)36-19(11-22(41)42)27(46)34-15-5-6-16-17(29(30,31)32)10-23(43)48-20(16)9-15/h5-6,9-10,12-13,18-19,24H,7-8,11H2,1-4H3, (H,33,38)(H,34,46)(H,35,44)(H,36,47)(H,37,45)(H,39,40)(H,41,42)

InChi Key: OGDZWRZGOFQIOD-UHFFFAOYSA-N