Caspase-8 Substrate IETD-AFC | 1062

Ready-to-use fluorometric substrate for FLICE/caspase-8 and related caspases that recognize the amino acid sequence IETD. The sequence IETD is based on caspase-8 cleavage site in CPP32/caspase-3 proenzyme. FLICE and related caspase activity can be quantified by fluorescent detection of free AFC after cleavage from the peptide substrate IETD-AFC at Ex. = 400 nm and Em. = 505 nm, using a fluorometer or multi-well fluorescence plate reader. Alternatively, a shift in fluorescence from blue to green upon cleavage can be visualized using a hand-held long-UV lamp.

IETD-AFC is a ready-to-use fluorometric substrate for FLICE/caspase-8 and related caspases that recognize the amino acid sequence IETD.

1062 | Caspase-8 Substrate IETD-AFC DataSheet

Alternate Name/Synonyms: Ac-IETD-AFC, Caspase-8 Substrate, Fluorogenic

Appearance: Liquid

Formulation: DMSO (2 mM)

CAS Number: N/A

Structure Available?: No

Peptide sequence: Ac-Ile-Glu-Thr-Asp-AFC

Salt Form: No

Molecular Formula: C₃₁H₃₈F₃N₅O₁₂

Molecular Weight: 729.7

Cell-Permeable?: No

Purity: >95% by HPLC

Solubilities: N/A

Handling: Protect from light and moisture

Country of Origin: USA

Tag Line: A Caspase-8 substrate

MDL Number: N/A

PubChem CID: 25108570

SMILES: CCC(C)C(C(=O)NC(CCC(=O)O)C(=O)NC(C(C)O)C(=O)NC(CC(=O)O)C(=O)NC1=CC2=C(C=C1)C(=CC(=O)O2)C(F)(F)F)NC(=O)C

InChi: InChI=1S/C31H38F3N5O12/c1-5-13(2)25(35-15(4)41)29(49)37-19(8-9-22(42)43)27(47)39-26(14(3)40)30(50)38-20(12-23(44)45)28(48)36-16-6-7-17-18(31(32,33)34)11-24(46)51-21(17)10-16/h6-7,10-11,13-14,19-20,25-26,40H,5,8-9,12H2,1-4H3,(H,35,41)(H,36,48)(H,37,49)(H,38,50)(H,39,47)(H,42,43)(H,44,45)/t13-,14+,19-,20-,25-,26-/m0/s1

InChi Key: CGUOWXRXLQXOKO-FCQBACSVSA-N