Caspase-8 Substrate IETD-pNA | 1063

Ready-to-use colorimetric substrate for FLICE/caspase-8 and related caspases that recognize the amino acid sequence IETD. The sequence IETD is based on caspase-8 cleavage site in CPP32/Caspase-3 proenzyme. FLICE and related caspase activity can be quantified by spectrophotometric detection of free pNA (l= 400 nm) after cleavage from the peptide substrate IETD-pNA, using a spectrophotometer or multi-well plate reader.

IETD-pNA is a ready-to-use fluorometric substrate for FLICE/caspase-8 and related caspases that recognize the amino acid sequence IETD.

1063 | Caspase-8 Substrate IETD-pNA DataSheet

Alternate Name/Synonyms: Ac-IETD-pNA, Caspase-8 Substrate, Colorimetric

Appearance: Liquid

Formulation: DMSO (4 mM)

CAS Number: N/A

Structure Available?: No

Peptide sequence: Ac-Ile-Glu-Thr-Asp-pNA

Salt Form: No

Molecular Formula: C₂₇H₃₈N₆O₁₂

Molecular Weight: 638.6

Cell-Permeable?: No

Purity: >95% by HPLC

Solubilities: N/A

Handling: Protect from light and moisture

Country of Origin: USA

Tag Line: A Caspase-8 substrate

MDL Number: MFCD01318840

PubChem CID: 25108782

SMILES: CCC(C)C(C(=O)NC(CCC(=O)O)C(=O)NC(C(C)O)C(=O)NC(CC(=O)O)C(=O)NC1=CC=C(C=C1)[N+](=O)[O-])NC(=O)C

InChi: InChI=1S/C27H38N6O12/c1-5-13(2)22(28-15(4)35)26(42)30-18(10-11-20(36)37)24(40)32-23(14(3)34)27(43)31-19(12-21(38)39)25(41)29-16-6-8-17(9-7-16)33(44)45/h6-9,13-14,18-19,22-23,34H,5,10-12H2,1-4H3,(H,28,35)(H,29,41)(H,30,42)(H,31,43)(H,32,40)(H,36,37)(H,38,39)/t13-,14+,18-,19-,22-,23-/m0/s1

InChi Key: FHURKTIGZAIQGR-BQCLNCLCSA-N