Caspase-12 Antibody (Large) | 3195

Caspase-12 Antibody (Large) | 3195 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: N/A

Immunogen: Caspase-12 antibody was raised against a synthetic peptide corresponding to 16 amino acids within the large cleavage product of murine caspase-12.
The immunogen is located within amino acids 170 - 220 of Caspase-12.

Research Area: Apoptosis, Cancer

Tested Application: E, WB, IHC-P, IF

Application: Caspase-12 (large) antibody can be used for the detection of both the propeptide and cleaved forms of caspase-12 by Western blot at 0.25 to 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 10 μg/mL.
Antibody validated: Western Blot in human, mouse, and rat samples; Immunohistochemistry in human, mouse, and rat samples and Immunofluorescence in human, mouse, and rat samples. All other applications and species not yet tested.

Specificiy: Caspase-12 (large) antibody is predicted to have no cross reactivity to other members in the caspase family.

Positive Control 1: Cat. No. 1301 - Human Heart Tissue Lysate

Positive Control 2: Cat. No. 1401 - Mouse Heart Tissue Lysate

Positive Control 3: Cat. No. 10-501 - Human Heart Tissue Slide

Positive Control 4: Cat. No. 1461 - Rat Heart Tissue Lysate

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 46 kDa
Observed: 46 kDa

Validation: N/A

Isoform: N/A

Purification: Caspase-12 (Large) Antibody is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Caspase-12 Antibody is supplied in PBS containing 0.02% sodium azide. (Large)

Concentration: 1 mg/mL

Storage Condition: Caspase-12 (Large) antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: Caspase-12 (Large) Antibody: Caspase-12, CASP-12

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: Caspase-12 Antibody: (Large) Three distinct signaling pathways lead to programmed cell death (apoptosis) . The death receptor and mitochondrion pathways are the main, in which the key apoptotic proteases capase-8 and caspase-9, respectively, are involved. The endoplasmic reticulum (ER) stress is the third apoptotic pathway and caspase-12 is involved. Caspase-12 is localized to the ER but not to cytoplasm or mitochondrion. Caspase-12 is activated by ER stress, including disruption of ER calcium homeostasis, and mediates ER stress-induced apoptosis. Caspase-12 is co-localized to the ER with several proteins that are involved in Alzheimer's disease including gamma-secretase presenilin and beta-amyloid precursor protein (APP) . Caspase-12 mediates cytotoxicity induced by amyloid-beta. Caspase-12 is ubiquitously expressed in mouse tissues.