Caspase-12 Antibody (Small) | 3197

Caspase-12 Antibody (Small) | 3197 | Gentaur UK, US & Europe Distribution

Host: Rabbit

Reactivity: Human, Mouse, Rat

Homology: Predicted species reactivity based on immunogen sequence: Bovine: (93%)

Immunogen: Caspase-12 antibody was raised against a synthetic peptide corresponding to 15 amino acids within the small cleavage product of murine caspase-12.
The immunogen is located within the last 50 amino acids of Caspase-12 .

Research Area: Apoptosis, Cancer

Tested Application: E, WB, IHC-P

Application: Caspase-12 (small) antibody can be used for the detection of both the propeptide and cleaved forms of caspase-12 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL.
Antibody validated: Western Blot in mouse and rat samples and Immunohistochemistry in human samples. All other applications and species not yet tested.

Specificiy: N/A

Positive Control 1: Cat. No. 1404 - Mouse Liver Tissue Lysate

Positive Control 2: Cat. No. 1464 - Rat Liver Tissue Lysate

Positive Control 3: Cat. No. 10-201 - Human Liver Tissue Slide

Positive Control 4: N/A

Positive Control 5: N/A

Positive Control 6: N/A

Molecular Weight: Predicted: 46 kDa (uncleaved)
Observed: 38 kDa

Validation: N/A

Isoform: N/A

Purification: Caspase-12 Antibody (Small) is affinity chromatography purified via peptide column.

Clonality: Polyclonal

Clone: N/A

Isotype: IgG

Conjugate: Unconjugated

Physical State: Liquid

Buffer: Caspase-12 Antibody is supplied in PBS containing 0.02% sodium azide. (Small)

Concentration: 1 mg/mL

Storage Condition: Caspase-12 antibody (Small) can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Alternate Name: Caspase-12 Antibody (Small) : Caspase-12, CASP-12

User Note: Optimal dilutions for each application to be determined by the researcher.

BACKGROUND: Caspase-12 Antibody: (Small) Three distinct signaling pathways lead to programmed cell death (apoptosis) . The death receptor and mitochondrion pathways are the main, in which the key apoptotic proteases capase-8 and caspase-9, respectively, are involved. The endoplasmic reticulum (ER) stress is the third apoptotic pathway and caspase-12 is involved. Caspase-12 is localized to the ER but not to cytoplasm or mitochondrion. Caspase-12 is activated by ER stress, including disruption of ER calcium homeostasis, and mediates ER stress-induced apoptosis. Caspase-12 is co-localized to the ER with several proteins that are involved in Alzheimer's disease including gamma-secretase presenilin and beta-amyloid precursor protein (APP) . Caspase-12 mediates cytotoxicity induced by amyloid-beta. Caspase-12 is ubiquitously expressed in mouse tissues.