Caspase-13 Substrate LEED-AFC | 1116

Ready-to-use fluorometric substrate for caspase-13 and related caspases that recognize the amino acid sequence LEED. Caspase-13 and related caspase activity can be quantified by fluorescent detection of free AFC after cleavage from the peptide substrate LEED-AFC at Ex. = 400 nm and Em. = 505 nm, using a fluorometer or multi-well fluorescence plate reader. Alternatively, a shift in fluorescence from blue to green upon cleavage can be visualized using a hand-held long-UV lamp.

LEED-AFC is a ready-to-use fluorometric substrate for caspase-13 and related caspases that recognize the amino acid sequence LEED.

1116 | Caspase-13 Substrate LEED-AFC DataSheet

Alternate Name/Synonyms: Ac-LEED-AFC, Caspase-13 Substrate, Fluorogenic

Appearance: Liquid

Formulation: DMSO (4 mM)

CAS Number: N/A

Structure Available?: No

Peptide sequence: Ac-Leu-Glu-Glu-Asp-AFC

Salt Form: No

Molecular Formula: C₃₂H₃₈F₃N₅O₁₃

Molecular Weight: 757.66

Cell-Permeable?: No

Purity: >98% by HPLC

Solubilities: N/A

Handling: Protect from light and moisture

Country of Origin: USA

Tag Line: A caspase-13 substrate

MDL Number: MFCD03452816

PubChem CID: 44135106

SMILES: CC(C)CC(C(=O)NC(CCC(=O)O)C(=O)NC(CCC(=O)O)C(=O)NC(CC(=O)O)C(=O)NC1=CC2=C(C=C1)C(=CC(=O)O2)C(F)(F)F)NC(=O)C

InChi: InChI=1S/C32H38F3N5O13/c1-14(2)10-21(36-15(3)41)31(52)39-19(6-8-24(42)43)28(49)38-20(7-9-25(44)45)29(50)40-22(13-26(46)47)30(51)37-16-4-5-17-18(32(33,34)35)12-27(48)53-23(17)11-16/h4-5,11-12,14,19-22H,6-10,13H2,1-3H3,(H,36,41)(H,37,51)(H,38,49)(H,39,52)(H,40,50)(H,42,43)(H,44,45)(H,46,47)

InChi Key: ZPNCQMJLTSFJQH-UHFFFAOYSA-N