Caspase-2 Substrate VDVAD-AFC | 1071

Ready-to-use fluorometric substrate for caspases that recognize the amino acid sequence VDVAD. Caspase activity can be quantified by fluorescent detection of free AFC after cleaved from the peptide substrate VDVAD-AFC at Ex/Em = 400/505 nm using a fluorometer or a fluorescence plate reader. Alternatively, a shift in fluorescence from blue to green upon cleavage can be visualized using a hand-held long-UV lamp. The ready-to-use caspase substrate provides an economic alternative for researchers who perform large amount of caspase assays. Cell Lysis Buffer (Cat.# 1067-100), 2X Reaction Buffer (Cat.# 1068-20), DTT (Cat.# 1201-1), and other reagents used for caspase activity assays are also available separately.

VDVAD-AFC is a ready-to-use fluorometric substrate for caspase-2 that recognize the amino acid sequence VDVAD.

1071 | Caspase-2 Substrate VDVAD-AFC DataSheet

Alternate Name/Synonyms: Ac-VDVAD-AFC, Caspase-2 Substrate, Fluorogenic, VDVAD-AFC, caspase2 substrate, caspase 2 substrate

Appearance: Liquid

Formulation: DMSO ( 1 mM)

CAS Number: N/A

Structure Available?: No

Peptide sequence: Ac-Val-Asp-Val-Ala-Asp-AFC

Salt Form: No

Molecular Formula: C₃₃H₄₁F₃N₆O₁₂

Molecular Weight: 770.7

Cell-Permeable?: No

Purity: >98% by HPLC

Solubilities: N/A

Handling: Protect from light and moisture

Country of Origin: USA

Tag Line: A caspase-2 substrate

MDL Number: MFCD04037014

PubChem CID: 44135100

SMILES: CC(C)C(C(=O)NC(CC(=O)O)C(=O)NC(C(C)C)C(=O)NC(C)C(=O)NC(CC(=O)O)C(=O)NC1=CC2=C(C=C1)C(=CC(=O)O2)C(F)(F)F)NC(=O)C

InChi: InChI=1S/C33H41F3N6O12/c1-13(2)26(38-16(6)43)32(53)41-21(12-24(46)47)30(51)42-27(14(3)4)31(52)37-15(5)28(49)40-20(11-23(44)45)29(50)39-17-7-8-18-19(33(34,35)36)10-25(48)54-22(18)9-17/h7-10,13-15,20-21,26-27H, 11-12H2,1-6H3,(H,37,52)(H,38,43)(H,39,50)(H,40,49)(H,41,53)(H,42,51)(H,44,45)(H,46,47)

InChi Key: BKQMJQIAIFEZJR-UHFFFAOYSA-N