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Caspase-3 Proform, mouse recombinant | 1183P
- SKU:
- 26-1183P-GEN
- Availability:
- Usually shipped in 5 working days
Description
Caspase-3 (also know as CPP32, Yama and apopain) is a member of the caspase family of cysteine proteases. Caspase-3 exists in cells as an inactive 32 kDa proenzyme. Proteolytic processing of this inactive proenzyme generates the 17 kD and 12 kD subunits which, when assembled as a tetramer (a pair of heterodimers), form the active caspase. Cascades of caspase activation have been shown to be key signal-transducing events in apoptosis. Procaspase-3, like other procaspases with short N-terminal prodomains, is considered to be a downstream or effector enzyme. Pro-caspase-3 can be cleaved and activated by caspase-8 and 10 which are themselves activated through interaction with death ligand receptor/adapter protein complexes (e.g., Fas/FADD). Procaspase-3 is also processed by caspase-9, the apical caspase that is activated by binding to the Apaf-1/cytochrome c/ATP apoptosome. Thus, activation of procaspase-3 stands at a point of convergence for the two major types of apoptosis signaling pathways-those linked to cell surface death receptors and those linked to mitochondrial release of cytochrome c. The recombinant Procaspase-3 (1183P-5) is produced by expression of a human cDNA in E. coli. The purified protein is highly activatable by treatment with active recombinant caspase-8 (1088-25, -100). We routinely test the activation using 10-30 ng of the procaspase-3 treated with 0.1-0.5 units of the active recombinant caspase-8 in 100 µl of Reaction Buffer (1068-20) for 30 minutes. Activation of procaspase-3 can be monitored using the Caspase-3 Fluorometric and Colorimetric Assay Kits (K105 and K106).
1183P | Caspase-3 Proform mouse recombinant DataSheet
Biomolecule/Target: Caspase-3
Synonyms: Caspase 3 Proform
Alternates names: Caspase-3, Short name=CASP-3, Apopain, Cysteine protease CPP32, Short name=CPP-32, FLICE, Protein Yama, SREBP cleavage activity 1, Short name=SCA-1
Taglines: A cysteine-aspartic acid protease involved in apoptosis
NCBI Gene ID #:
NCBI Gene Symbol:
Gene Source: Mouse
Accession #:
Recombinant: Yes
Source: E. coli
Purity by SDS-PAGEs: N/A
Assay: SDS-PAGE
Purity: N/A
Assay #2: HPLC
Endotoxin Level: N/A
Activity (Specifications/test method): N/A
Biological activity: N/A
Results: N/A
Binding Capacity: N/A
Unit Definition: N/A
Molecular Weight: 32.0 kDa
Concentration: N/A
Appearance: Lyophilized powder
Physical form description: Lyophilized powder
Reconstitution Instructions: Reconstitute to 0.1 µg/µl in PBS containing 15% glycerol.
Amino acid sequence: N/A